After transfer is complete, the membrane must undergo thorough blocking. The membrane contains non-specific protein binding sites. To prevent these sites from binding to antibodies and causing non-specific staining and background, inert proteins or non-ionic detergents are typically used to block the unbound sites on the membrane, thereby reducing non-specific antibody binding. The blocking agent should block all unbound sites without replacing the target protein on the membrane, binding to the epitope of the target protein, or cross-reacting with antibodies or detection reagents.
Cat. No. | Description | Packaging |
BL1315A | Western Solution Set (Blocking Buffer, Wash Buffer, Primary and Secondary Antibody Diluents) | 4×100mL |
BL535A | Western Blocking Buffer | 100mL |
BL1032A | Western Protein-Free Rapid Blocking Buffer | 100mL |
BL506A | Western Primary Antibody Diluent | 100mL |
BL506B | Western Primary Antibody Diluent | 500mL |
BL1027A | Western Protein-Free Primary Antibody Diluent | 100mL |
BL1027B | Western Protein-Free Primary Antibody Diluent | 500mL |
BL536A | Western Protein-Free Secondary Antibody Diluent | 100mL |
BL536B | Western Protein-Free Secondary Antibody Diluent | 500mL |
BL1060A | Universal Antibody Diluent | 100mL |
BL1060B | Universal Antibody Diluent | 500mL |
After transfer is complete, the membrane must undergo thorough blocking. The membrane contains non-specific protein binding sites. To prevent these sites from binding to antibodies and causing non-specific staining and background, inert proteins or non-ionic detergents are typically used to block the unbound sites on the membrane, thereby reducing non-specific antibody binding. The blocking agent should block all unbound sites without replacing the target protein on the membrane, binding to the epitope of the target protein, or cross-reacting with antibodies or detection reagents.
Cat. No. | Description | Packaging |
BL1315A | Western Solution Set (Blocking Buffer, Wash Buffer, Primary and Secondary Antibody Diluents) | 4×100mL |
BL535A | Western Blocking Buffer | 100mL |
BL1032A | Western Protein-Free Rapid Blocking Buffer | 100mL |
BL506A | Western Primary Antibody Diluent | 100mL |
BL506B | Western Primary Antibody Diluent | 500mL |
BL1027A | Western Protein-Free Primary Antibody Diluent | 100mL |
BL1027B | Western Protein-Free Primary Antibody Diluent | 500mL |
BL536A | Western Protein-Free Secondary Antibody Diluent | 100mL |
BL536B | Western Protein-Free Secondary Antibody Diluent | 500mL |
BL1060A | Universal Antibody Diluent | 100mL |
BL1060B | Universal Antibody Diluent | 500mL |
