Polyacrylamide gel electrophoresis (PAGE) is based on the mesh-like structure of polyacrylamide gel, which acts as a molecular sieve. Under an electric current, protein samples migrate from the top to the bottom of the gel. Larger proteins migrate more slowly, while smaller proteins migrate faster. Protein sizes can be identified by comparison with protein markers. Polyacrylamide gel electrophoresis includes two forms: native-PAGE and SDS-PAGE. In native-PAGE, proteins remain in their native state during electrophoresis and are separated based on molecular weight, shape, and charge. SDS-PAGE is the most commonly used protein expression analysis technique in polyacrylamide gel electrophoresis. SDS is an anionic detergent that binds to proteins and disrupts intra- and intermolecular hydrogen bonds, destroying secondary and tertiary structures and forming negatively charged ellipsoidal complexes. The charge on these complexes is significantly greater than the original charge on the proteins, largely eliminating differences in native charge and shape. As a result, migration rates in SDS-PAGE depend primarily on protein molecular weight. Due to its high sensitivity, SDS-PAGE is also commonly used to assess purity during purification processes. A critical step in protein gel electrophoresis is gel preparation, which typically involves acrylamide/bis-acrylamide solution, Tris-HCl, 10% SDS, the catalyst 10% APS, and the accelerator TEMED. Various preparation methods and products are available, ranging from traditional gel casting to precast gels, meeting the diverse needs of researchers.
Gel Preparation Reagents
Traditional gel preparation requires purchasing individual components for gel preparation according to the classic formula. This approach is cost-effective and allows preparation of gels at different concentrations as needed. However, it involves many reagents, complex calculations, tedious operations, and is time-consuming, with the risk of uneven or unsuccessful gel preparation. Additionally, reagents such as acrylamide solution and TEMED are toxic, posing potential safety concerns for experimenters.
| Cat. No. | Description | Packaging |
| BL513B | 30% Acr-Bis (29:1) Gel Solution | 500mL |
| BL667A | 40% Acr-Bis (29:1) Gel Solution | 100mL |
| BL667B | 40% Acr-Bis (29:1) Gel Solution | 500mL |
| BL668A | 40% Acr-Bis (37.5:1) Gel Solution | 100mL |
| BL668B | 40% Acr-Bis (37.5:1) Gel Solution | 500mL |
| BL669A | 40% Acr-Bis (19:1) Gel Solution | 100mL |
| BL669B | 40% Acr-Bis (19:1) Gel Solution | 500mL |
| BL514A | 1 M Tris-HCl Solution, pH 6.8 | 100mL |
| BL514B | 1 M Tris-HCl Solution, pH 6.8 | 500mL |
| BL515A | 1 M Tris-HCl Solution, pH 8.8 | 100mL |
| BL515B | 1 M Tris-HCl Solution, pH 8.8 | 500mL |
| BL516A | 1.5 M Tris-HCl Solution, pH 8.8 | 100mL |
| BL516B | 1.5 M Tris-HCl Solution, pH 8.8 | 500mL |
| BL517A | 10% SDS Solution | 100mL |
| BL517B | 10% SDS Solution | 500mL |
| BL1222A | 4× Tris/SDS Resolving Gel Buffer, pH 8.8 | 100mL |
| BL1222B | 4× Tris/SDS Resolving Gel Buffer, pH 8.8 | 500mL |
| BL1223A | 4× Tris/SDS Stacking Gel Buffer, pH 6.8 | 100mL |
| BL1223B | 4× Tris/SDS Stacking Gel Buffer, pH 6.8 | 500mL |
Gel Preparation Kit
The gel preparation kit includes all the reagents required for gel preparation. No additional reagents need to be purchased. The components are designed to work together for consistent results. Based on the molecular weight of the target protein, gels of different concentrations can be prepared according to the formulation table, meeting various experimental needs.
| Cat. No. | Description | Packaging |
| BL508A | SDS-PAGE Gel Preparation Kit | 50T |
| BL522A | SDS-PAGE Gel Rapid Preparation Kit | 50T |
| BL1336A | Native-PAGE Gel Preparation Kit | 50T |
Rapid Gel Preparation Kit
The rapid gel preparation kit features optimized mixtures of components for different gel concentrations (6%, 8%, 10%, 12%, 15%), simplifying the number of pipetting steps during gel preparation. To cast a gel, simply add appropriate volumes of resolving/stacking gel solution and resolving/stacking gel buffer in a 1:1 ratio, then add the polymerization initiator. Compared to the previous two methods, this product eliminates the need to calculate individual component volumes, making gel preparation more convenient.
- Rapid gel casting: Multiple gels can be cast in a short time without the need to calculate required solution volumes or perform dilutions
- Colored stacking gel: Allows preparation of red-colored stacking gels, providing maximum convenience for sample loading and gel differentiation; separate stacking gel dye also available for individual order
- Odor-free: Improved formulation eliminates the need for TEMED, with no unpleasant odor
| Cat. No. | Description | Packaging |
| BL563B | 6% PAGE Color Gel Rapid Preparation Kit (Light Yellow) | 125 sheets/box |
| BL564B | 8% PAGE Color Gel Rapid Preparation Kit (Light Green) | 125 sheets/box |
| BL565B | 10% PAGE Color Gel Rapid Preparation Kit (Light Blue) | 125 sheets/box |
| BL566B | 12% PAGE Color Gel Rapid Preparation Kit (Light Red) | 125 sheets/box |
| BL567B | 15% PAGE Color Gel Rapid Preparation Kit (Light Purple) | 125 sheets/box |
| BL1464A | 6% PAGE Color Gel Rapid Preparation Kit (Seal-Free) (Light Yellow) | 125 sheets/box |
| BL1465A | 8% PAGE Color Gel Rapid Preparation Kit (Seal-Free) (Light Green) | 125 sheets/box |
| BL1466A | 10% PAGE Color Gel Rapid Preparation Kit (Seal-Free) (Light Blue) | 125 sheets/box |
| BL1467A | 12% PAGE Color Gel Rapid Preparation Kit (Seal-Free) (Light Red) | 125 sheets/box |
| BL1468A | 15% PAGE Color Gel Rapid Preparation Kit (Seal-Free) (Light Purple) | 125 sheets/box |
| BL584A | PAGE Stacking Gel Dye (250×, Red) | 1mL |
| BL584B | PAGE Stacking Gel Dye (250×, Yellow) | 1mL |
| BL584C | PAGE Stacking Gel Dye (250×, Blue) | 1mL |
| BL584D | PAGE Stacking Gel Dye (250×, Green) | 1mL |
| BL584E | PAGE Stacking Gel Dye (250×, Purple) | 1mL |
Colored Stacking Gel
When preparing the stacking gel, add the appropriate colored dye, mix well, and let it sit until solidified. This dye makes the stacking gel wells clearly visible, allowing accurate sample loading and easy identification of well edges or any distortion or damage. The dye does not migrate into the resolving gel during electrophoresis and remains stably in the stacking gel, facilitating easy identification and removal of the stacking gel after electrophoresis without affecting electrophoresis or staining results.
Polyacrylamide gel electrophoresis (PAGE) is based on the mesh-like structure of polyacrylamide gel, which acts as a molecular sieve. Under an electric current, protein samples migrate from the top to the bottom of the gel. Larger proteins migrate more slowly, while smaller proteins migrate faster. Protein sizes can be identified by comparison with protein markers. Polyacrylamide gel electrophoresis includes two forms: native-PAGE and SDS-PAGE. In native-PAGE, proteins remain in their native state during electrophoresis and are separated based on molecular weight, shape, and charge. SDS-PAGE is the most commonly used protein expression analysis technique in polyacrylamide gel electrophoresis. SDS is an anionic detergent that binds to proteins and disrupts intra- and intermolecular hydrogen bonds, destroying secondary and tertiary structures and forming negatively charged ellipsoidal complexes. The charge on these complexes is significantly greater than the original charge on the proteins, largely eliminating differences in native charge and shape. As a result, migration rates in SDS-PAGE depend primarily on protein molecular weight. Due to its high sensitivity, SDS-PAGE is also commonly used to assess purity during purification processes. A critical step in protein gel electrophoresis is gel preparation, which typically involves acrylamide/bis-acrylamide solution, Tris-HCl, 10% SDS, the catalyst 10% APS, and the accelerator TEMED. Various preparation methods and products are available, ranging from traditional gel casting to precast gels, meeting the diverse needs of researchers.
Gel Preparation Reagents
Traditional gel preparation requires purchasing individual components for gel preparation according to the classic formula. This approach is cost-effective and allows preparation of gels at different concentrations as needed. However, it involves many reagents, complex calculations, tedious operations, and is time-consuming, with the risk of uneven or unsuccessful gel preparation. Additionally, reagents such as acrylamide solution and TEMED are toxic, posing potential safety concerns for experimenters.
| Cat. No. | Description | Packaging |
| BL513B | 30% Acr-Bis (29:1) Gel Solution | 500mL |
| BL667A | 40% Acr-Bis (29:1) Gel Solution | 100mL |
| BL667B | 40% Acr-Bis (29:1) Gel Solution | 500mL |
| BL668A | 40% Acr-Bis (37.5:1) Gel Solution | 100mL |
| BL668B | 40% Acr-Bis (37.5:1) Gel Solution | 500mL |
| BL669A | 40% Acr-Bis (19:1) Gel Solution | 100mL |
| BL669B | 40% Acr-Bis (19:1) Gel Solution | 500mL |
| BL514A | 1 M Tris-HCl Solution, pH 6.8 | 100mL |
| BL514B | 1 M Tris-HCl Solution, pH 6.8 | 500mL |
| BL515A | 1 M Tris-HCl Solution, pH 8.8 | 100mL |
| BL515B | 1 M Tris-HCl Solution, pH 8.8 | 500mL |
| BL516A | 1.5 M Tris-HCl Solution, pH 8.8 | 100mL |
| BL516B | 1.5 M Tris-HCl Solution, pH 8.8 | 500mL |
| BL517A | 10% SDS Solution | 100mL |
| BL517B | 10% SDS Solution | 500mL |
| BL1222A | 4× Tris/SDS Resolving Gel Buffer, pH 8.8 | 100mL |
| BL1222B | 4× Tris/SDS Resolving Gel Buffer, pH 8.8 | 500mL |
| BL1223A | 4× Tris/SDS Stacking Gel Buffer, pH 6.8 | 100mL |
| BL1223B | 4× Tris/SDS Stacking Gel Buffer, pH 6.8 | 500mL |
Gel Preparation Kit
The gel preparation kit includes all the reagents required for gel preparation. No additional reagents need to be purchased. The components are designed to work together for consistent results. Based on the molecular weight of the target protein, gels of different concentrations can be prepared according to the formulation table, meeting various experimental needs.
| Cat. No. | Description | Packaging |
| BL508A | SDS-PAGE Gel Preparation Kit | 50T |
| BL522A | SDS-PAGE Gel Rapid Preparation Kit | 50T |
| BL1336A | Native-PAGE Gel Preparation Kit | 50T |
Rapid Gel Preparation Kit
The rapid gel preparation kit features optimized mixtures of components for different gel concentrations (6%, 8%, 10%, 12%, 15%), simplifying the number of pipetting steps during gel preparation. To cast a gel, simply add appropriate volumes of resolving/stacking gel solution and resolving/stacking gel buffer in a 1:1 ratio, then add the polymerization initiator. Compared to the previous two methods, this product eliminates the need to calculate individual component volumes, making gel preparation more convenient.
- Rapid gel casting: Multiple gels can be cast in a short time without the need to calculate required solution volumes or perform dilutions
- Colored stacking gel: Allows preparation of red-colored stacking gels, providing maximum convenience for sample loading and gel differentiation; separate stacking gel dye also available for individual order
- Odor-free: Improved formulation eliminates the need for TEMED, with no unpleasant odor
| Cat. No. | Description | Packaging |
| BL563B | 6% PAGE Color Gel Rapid Preparation Kit (Light Yellow) | 125 sheets/box |
| BL564B | 8% PAGE Color Gel Rapid Preparation Kit (Light Green) | 125 sheets/box |
| BL565B | 10% PAGE Color Gel Rapid Preparation Kit (Light Blue) | 125 sheets/box |
| BL566B | 12% PAGE Color Gel Rapid Preparation Kit (Light Red) | 125 sheets/box |
| BL567B | 15% PAGE Color Gel Rapid Preparation Kit (Light Purple) | 125 sheets/box |
| BL1464A | 6% PAGE Color Gel Rapid Preparation Kit (Seal-Free) (Light Yellow) | 125 sheets/box |
| BL1465A | 8% PAGE Color Gel Rapid Preparation Kit (Seal-Free) (Light Green) | 125 sheets/box |
| BL1466A | 10% PAGE Color Gel Rapid Preparation Kit (Seal-Free) (Light Blue) | 125 sheets/box |
| BL1467A | 12% PAGE Color Gel Rapid Preparation Kit (Seal-Free) (Light Red) | 125 sheets/box |
| BL1468A | 15% PAGE Color Gel Rapid Preparation Kit (Seal-Free) (Light Purple) | 125 sheets/box |
| BL584A | PAGE Stacking Gel Dye (250×, Red) | 1mL |
| BL584B | PAGE Stacking Gel Dye (250×, Yellow) | 1mL |
| BL584C | PAGE Stacking Gel Dye (250×, Blue) | 1mL |
| BL584D | PAGE Stacking Gel Dye (250×, Green) | 1mL |
| BL584E | PAGE Stacking Gel Dye (250×, Purple) | 1mL |
Colored Stacking Gel
When preparing the stacking gel, add the appropriate colored dye, mix well, and let it sit until solidified. This dye makes the stacking gel wells clearly visible, allowing accurate sample loading and easy identification of well edges or any distortion or damage. The dye does not migrate into the resolving gel during electrophoresis and remains stably in the stacking gel, facilitating easy identification and removal of the stacking gel after electrophoresis without affecting electrophoresis or staining results.
