Polyacrylamide gel electrophoresis (PAGE) is based on the mesh-like structure of polyacrylamide gel, which acts as a molecular sieve. Under an electric current, protein samples migrate from the top to the bottom of the gel. Larger proteins migrate more slowly, while smaller proteins migrate faster. Protein sizes can be identified by comparison with protein markers. Polyacrylamide gel electrophoresis includes two forms: native-PAGE and SDS-PAGE. In native-PAGE, proteins remain in their native state during electrophoresis and are separated based on molecular weight, shape, and charge. SDS-PAGE is the most commonly used protein expression analysis technique in polyacrylamide gel electrophoresis. SDS is an anionic detergent that binds to proteins and disrupts intra- and intermolecular hydrogen bonds, destroying secondary and tertiary structures and forming negatively charged ellipsoidal complexes. The charge on these complexes is significantly greater than the original charge on the proteins, largely eliminating differences in native charge and shape. As a result, migration rates in SDS-PAGE depend primarily on protein molecular weight. Due to its high sensitivity, SDS-PAGE is also commonly used to assess purity during purification processes. A critical step in protein gel electrophoresis is gel preparation, which typically involves acrylamide/bis-acrylamide solution, Tris-HCl, 10% SDS, the catalyst 10% APS, and the accelerator TEMED. Various preparation methods and products are available, ranging from traditional gel casting to precast gels, meeting the diverse needs of researchers.
Gel Preparation Reagents
Traditional gel preparation requires purchasing individual components for gel preparation according to the classic formula. This approach is cost-effective and allows preparation of gels at different concentrations as needed. However, it involves many reagents, complex calculations, tedious operations, and is time-consuming, with the risk of uneven or unsuccessful gel preparation. Additionally, reagents such as acrylamide solution and TEMED are toxic, posing potential safety concerns for experimenters.
| Cat. No. | Description | Packaging |
| BL514A | 1 M Tris-HCl Solution, pH 6.8 | 100mL |
| BL514B | 1 M Tris-HCl Solution, pH 6.8 | 500mL |
| BL515A | 1 M Tris-HCl Solution, pH 8.8 | 100mL |
| BL515B | 1 M Tris-HCl Solution, pH 8.8 | 500mL |
| BL516A | 1.5 M Tris-HCl Solution, pH 8.8 | 100mL |
| BL516B | 1.5 M Tris-HCl Solution, pH 8.8 | 500mL |
| BL517A | 10% SDS Solution | 100mL |
| BL517B | 10% SDS Solution | 500mL |
| BL1222A | 4× Tris/SDS Resolving Gel Buffer, pH 8.8 | 100mL |
| BL1222B | 4× Tris/SDS Resolving Gel Buffer, pH 8.8 | 500mL |
| BL1223A | 4× Tris/SDS Stacking Gel Buffer, pH 6.8 | 100mL |
| BL1223B | 4× Tris/SDS Stacking Gel Buffer, pH 6.8 | 500mL |
Polyacrylamide gel electrophoresis (PAGE) is based on the mesh-like structure of polyacrylamide gel, which acts as a molecular sieve. Under an electric current, protein samples migrate from the top to the bottom of the gel. Larger proteins migrate more slowly, while smaller proteins migrate faster. Protein sizes can be identified by comparison with protein markers. Polyacrylamide gel electrophoresis includes two forms: native-PAGE and SDS-PAGE. In native-PAGE, proteins remain in their native state during electrophoresis and are separated based on molecular weight, shape, and charge. SDS-PAGE is the most commonly used protein expression analysis technique in polyacrylamide gel electrophoresis. SDS is an anionic detergent that binds to proteins and disrupts intra- and intermolecular hydrogen bonds, destroying secondary and tertiary structures and forming negatively charged ellipsoidal complexes. The charge on these complexes is significantly greater than the original charge on the proteins, largely eliminating differences in native charge and shape. As a result, migration rates in SDS-PAGE depend primarily on protein molecular weight. Due to its high sensitivity, SDS-PAGE is also commonly used to assess purity during purification processes. A critical step in protein gel electrophoresis is gel preparation, which typically involves acrylamide/bis-acrylamide solution, Tris-HCl, 10% SDS, the catalyst 10% APS, and the accelerator TEMED. Various preparation methods and products are available, ranging from traditional gel casting to precast gels, meeting the diverse needs of researchers.
Gel Preparation Reagents
Traditional gel preparation requires purchasing individual components for gel preparation according to the classic formula. This approach is cost-effective and allows preparation of gels at different concentrations as needed. However, it involves many reagents, complex calculations, tedious operations, and is time-consuming, with the risk of uneven or unsuccessful gel preparation. Additionally, reagents such as acrylamide solution and TEMED are toxic, posing potential safety concerns for experimenters.
| Cat. No. | Description | Packaging |
| BL514A | 1 M Tris-HCl Solution, pH 6.8 | 100mL |
| BL514B | 1 M Tris-HCl Solution, pH 6.8 | 500mL |
| BL515A | 1 M Tris-HCl Solution, pH 8.8 | 100mL |
| BL515B | 1 M Tris-HCl Solution, pH 8.8 | 500mL |
| BL516A | 1.5 M Tris-HCl Solution, pH 8.8 | 100mL |
| BL516B | 1.5 M Tris-HCl Solution, pH 8.8 | 500mL |
| BL517A | 10% SDS Solution | 100mL |
| BL517B | 10% SDS Solution | 500mL |
| BL1222A | 4× Tris/SDS Resolving Gel Buffer, pH 8.8 | 100mL |
| BL1222B | 4× Tris/SDS Resolving Gel Buffer, pH 8.8 | 500mL |
| BL1223A | 4× Tris/SDS Stacking Gel Buffer, pH 6.8 | 100mL |
| BL1223B | 4× Tris/SDS Stacking Gel Buffer, pH 6.8 | 500mL |
