In Vitro Transcription (IVT) is a core technology for efficient RNA synthesis in cell-free systems. Using specific promoters (such as T7, T3, or SP6) and corresponding RNA polymerases, it produces high-purity target RNA from linearized DNA templates under optimized reaction conditions. IVT products are widely used in life science research and biopharmaceutical applications, including mRNA vaccines and therapeutics, development of therapeutic RNA molecules (e.g., siRNA, saRNA), RNA functional studies, research reagent preparation, and in vitro protein synthesis. With its high efficiency, flexibility, and scalability, this technology serves as a key platform driving cutting-edge research and innovative therapy development.
In Vitro Transcription Kit
The T7 In Vitro Transcription Kit utilizes an optimized reaction system with T7 RNA Polymerase to perform in vitro transcription. Using linear double-stranded DNA containing the T7 promoter sequence as a template and NTPs as substrates, it transcribes DNA sequences downstream of the promoter, enabling simple and rapid production of large quantities of RNA. This product series can also generate biotin-, dye-, or radiolabeled RNA using modified nucleotides, as well as capped RNA using cap structures or cap analogs as substrates.
Cat. No. | Description | Packaging |
BL5660A | High-Yield T7 In Vitro Transcription Kit (N1-Me-pUTP) | 50T |
BL5661A | High-Yield T7 In Vitro Transcription Kit (pUTP) | 50T |
BL5662A | Co-Transcriptional Capping T7 In Vitro Transcription Kit (pUTP, CAP GAG (3OMe)) | 50T |
BL5663A | Co-Transcriptional Capping T7 In Vitro Transcription Kit (pUTP, CAP GAG) | 50T |
BL5664A | Co-Transcriptional Capping T7 In Vitro Transcription Kit (N1-Me-pUTP, CAP GAG (3OMe)) | 50T |
BL5665A | Co-Transcriptional Capping T7 In Vitro Transcription Kit (N1-Me-pUTP, CAP GAG) | 50T |
BL5666A | High-Yield T7 In Vitro Transcription Kit (Low dsRNA) | 50T |
BL5667A | High-Yield T7 In Vitro Transcription Kit (High-Temperature Resistant) | 50T |
Enzymatic Capping Kit
This kit contains Vaccinia Capping Enzyme, mRNA Cap 2´-O-Methyltransferase, and other capping-related components, enabling the addition of cap0 or cap1 structures to the 5' end of uncapped mRNA. This structure is identical to the natural cap found in eukaryotic cells, significantly reducing the immunogenicity of exogenous mRNA, enhancing its stability, and improving translation efficiency.
Cat. No. | Description | Packaging |
BL5669A | Enzymatic Capping Kit | 50T |
mRNA Modification Reagent
This product is used during in vitro transcription to add a cap structure to the 5' end of mRNA, forming a Cap 1 structure. Compared to Cap 0 structures (ARCA and mCap capping), mRNA with a Cap 1 structure is better suited for mammalian systems and exhibits higher translational efficiency.
| Cat. No. | Description | Packaging |
| BL5671A | Cap Analog cap1 (3'OH AG) | 100μL |
| BL5671B | Cap Analog cap1 (3'OH AG) | 1mL |
| BL5671C | Cap Analog cap1 (3'OH AG) | 10mL |
| BL5671D | Cap Analog cap1 (3'OH AG) | 100mL |
| BL5672A | Cap Analog cap1 (3'OMe AG) | 100μL |
| BL5672B | Cap Analog cap1 (3'OMe AG) | 1mL |
| BL5672C | Cap Analog cap1 (3'OMe AG) | 10mL |
| BL5672D | Cap Analog cap1 (3'OMe AG) | 100mL |
In Vitro Transcription (IVT) is a core technology for efficient RNA synthesis in cell-free systems. Using specific promoters (such as T7, T3, or SP6) and corresponding RNA polymerases, it produces high-purity target RNA from linearized DNA templates under optimized reaction conditions. IVT products are widely used in life science research and biopharmaceutical applications, including mRNA vaccines and therapeutics, development of therapeutic RNA molecules (e.g., siRNA, saRNA), RNA functional studies, research reagent preparation, and in vitro protein synthesis. With its high efficiency, flexibility, and scalability, this technology serves as a key platform driving cutting-edge research and innovative therapy development.
In Vitro Transcription Kit
The T7 In Vitro Transcription Kit utilizes an optimized reaction system with T7 RNA Polymerase to perform in vitro transcription. Using linear double-stranded DNA containing the T7 promoter sequence as a template and NTPs as substrates, it transcribes DNA sequences downstream of the promoter, enabling simple and rapid production of large quantities of RNA. This product series can also generate biotin-, dye-, or radiolabeled RNA using modified nucleotides, as well as capped RNA using cap structures or cap analogs as substrates.
Cat. No. | Description | Packaging |
BL5660A | High-Yield T7 In Vitro Transcription Kit (N1-Me-pUTP) | 50T |
BL5661A | High-Yield T7 In Vitro Transcription Kit (pUTP) | 50T |
BL5662A | Co-Transcriptional Capping T7 In Vitro Transcription Kit (pUTP, CAP GAG (3OMe)) | 50T |
BL5663A | Co-Transcriptional Capping T7 In Vitro Transcription Kit (pUTP, CAP GAG) | 50T |
BL5664A | Co-Transcriptional Capping T7 In Vitro Transcription Kit (N1-Me-pUTP, CAP GAG (3OMe)) | 50T |
BL5665A | Co-Transcriptional Capping T7 In Vitro Transcription Kit (N1-Me-pUTP, CAP GAG) | 50T |
BL5666A | High-Yield T7 In Vitro Transcription Kit (Low dsRNA) | 50T |
BL5667A | High-Yield T7 In Vitro Transcription Kit (High-Temperature Resistant) | 50T |
Enzymatic Capping Kit
This kit contains Vaccinia Capping Enzyme, mRNA Cap 2´-O-Methyltransferase, and other capping-related components, enabling the addition of cap0 or cap1 structures to the 5' end of uncapped mRNA. This structure is identical to the natural cap found in eukaryotic cells, significantly reducing the immunogenicity of exogenous mRNA, enhancing its stability, and improving translation efficiency.
Cat. No. | Description | Packaging |
BL5669A | Enzymatic Capping Kit | 50T |
mRNA Modification Reagent
This product is used during in vitro transcription to add a cap structure to the 5' end of mRNA, forming a Cap 1 structure. Compared to Cap 0 structures (ARCA and mCap capping), mRNA with a Cap 1 structure is better suited for mammalian systems and exhibits higher translational efficiency.
| Cat. No. | Description | Packaging |
| BL5671A | Cap Analog cap1 (3'OH AG) | 100μL |
| BL5671B | Cap Analog cap1 (3'OH AG) | 1mL |
| BL5671C | Cap Analog cap1 (3'OH AG) | 10mL |
| BL5671D | Cap Analog cap1 (3'OH AG) | 100mL |
| BL5672A | Cap Analog cap1 (3'OMe AG) | 100μL |
| BL5672B | Cap Analog cap1 (3'OMe AG) | 1mL |
| BL5672C | Cap Analog cap1 (3'OMe AG) | 10mL |
| BL5672D | Cap Analog cap1 (3'OMe AG) | 100mL |
