This strain is generated by introducing theF´{lacIq Tn10 (TetR)} factor into the TOP10 strain, yielding TOP10F´. The F´ factor carries the lacIq repressor, which can suppress expression of genes downstream of promoters such as trc, tac, and lac, making it suitable for propagating plasmids expressing toxic proteins. Mutations in recA1 and endA1 facilitate stable insertion of DNA and high-purity plasmid DNA extraction. It can be used for cloning and blue-white screening (for blue-white screening, IPTG should be added to the medium to induce β-galactosidase expression). This strain exhibits tetracycline resistance.
- Chemical transformation efficiency >108 cfu/μg DNA
- Genotype：F´{lacIq Tn10 (TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK
rpsL endA1 - nupG
Cat. No. | Description | Packaging |
BL1878A | TOP10F` Competent Cells | 10×100 μL |
This strain is generated by introducing theF´{lacIq Tn10 (TetR)} factor into the TOP10 strain, yielding TOP10F´. The F´ factor carries the lacIq repressor, which can suppress expression of genes downstream of promoters such as trc, tac, and lac, making it suitable for propagating plasmids expressing toxic proteins. Mutations in recA1 and endA1 facilitate stable insertion of DNA and high-purity plasmid DNA extraction. It can be used for cloning and blue-white screening (for blue-white screening, IPTG should be added to the medium to induce β-galactosidase expression). This strain exhibits tetracycline resistance.
- Chemical transformation efficiency >108 cfu/μg DNA
- Genotype：F´{lacIq Tn10 (TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK
rpsL endA1 - nupG
Cat. No. | Description | Packaging |
BL1878A | TOP10F` Competent Cells | 10×100 μL |
