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TG1 is one of the fastest-growing E. coli strains for cloning, with colonies visible on plates after 7 h at 37°C. It is a primary strain for phage display and can also be used for general plasmid construction. The presence of lacIqZΔM15 enables blue-white screening. However, it does not carry the endA1 mutation and has high intracellular nuclease activity; therefore, it is recommended to use protein removal solution in plasmid extraction kits to eliminate the abundant nucleases during plasmid preparation.
- Chemical transformation efficiency >109 cfu/μg DNA
- Genotype:[F´ traD36 proAB lacIqZΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK‒mK‒)
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Cat. No. | Description | Packaging |
BL1872A | TG1 Competent Cells | 10×100 μL |
TG1 is one of the fastest-growing E. coli strains for cloning, with colonies visible on plates after 7 h at 37°C. It is a primary strain for phage display and can also be used for general plasmid construction. The presence of lacIqZΔM15 enables blue-white screening. However, it does not carry the endA1 mutation and has high intracellular nuclease activity; therefore, it is recommended to use protein removal solution in plasmid extraction kits to eliminate the abundant nucleases during plasmid preparation.
- Chemical transformation efficiency >109 cfu/μg DNA
- Genotype:[F´ traD36 proAB lacIqZΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK‒mK‒)
.
Cat. No. | Description | Packaging |
BL1872A | TG1 Competent Cells | 10×100 μL |