This strain is recommended for use in retrovirus/lentivirus vector systems and is particularly suitable for cloning lentiviral or DNA fragments with terminal repeats. The recA1 relA1 mutations effectively suppress recombination at terminal repeats of long fragments, reducing the probability of incorrect recombination. The endA1 mutation prevents nuclease contamination during plasmid extraction, significantly improving the yield and quality of high-purity viral plasmids. The presence of lacZΔM15 enables blue-white screening in Stable. This strain exhibits tetracycline and streptomycin resistance.
- Chemical transformation efficiency >5×108 cfu/μg DNA
- Genotype：F' proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (TetR) Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- Φ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS -mcrBC
Cat. No. | Description | Packaging |
BL1870A | Stable Competent Cells | 10×100 μL |
This strain is recommended for use in retrovirus/lentivirus vector systems and is particularly suitable for cloning lentiviral or DNA fragments with terminal repeats. The recA1 relA1 mutations effectively suppress recombination at terminal repeats of long fragments, reducing the probability of incorrect recombination. The endA1 mutation prevents nuclease contamination during plasmid extraction, significantly improving the yield and quality of high-purity viral plasmids. The presence of lacZΔM15 enables blue-white screening in Stable. This strain exhibits tetracycline and streptomycin resistance.
- Chemical transformation efficiency >5×108 cfu/μg DNA
- Genotype：F' proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (TetR) Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- Φ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS -mcrBC
Cat. No. | Description | Packaging |
BL1870A | Stable Competent Cells | 10×100 μL |
