This strain is derived from JM109 and is used for high-level expression of genes cloned into expression vectors containing the T7 promoter (e.g., pET series). The λ phage DE3 region, which contains the T7 RNA polymerase gene, is integrated into the chromosome of JM109, hence the name JM109(DE3). It expresses both T7 RNA polymerase and *E. coli* RNA polymerase and is suitable for protein expression using pET, pGEX, and pMAL plasmids. The JM109(DE3) strain cannot be used for blue-white screening.
- Chemical transformation efficiency >108 cfu /μg DNA
- Genotype：endA1 recA1 gyrA96 thi-1 hsdR17 (rk-,mk+) relA1 supE44 D (lac-proAB) [F´ traD36 proAB laqI qZΔM15](DE3)
Cat. No. | Description | Packaging |
BL1863A | JM109 (DE3) Competent Cells | 10×100 μL |
This strain is derived from JM109 and is used for high-level expression of genes cloned into expression vectors containing the T7 promoter (e.g., pET series). The λ phage DE3 region, which contains the T7 RNA polymerase gene, is integrated into the chromosome of JM109, hence the name JM109(DE3). It expresses both T7 RNA polymerase and *E. coli* RNA polymerase and is suitable for protein expression using pET, pGEX, and pMAL plasmids. The JM109(DE3) strain cannot be used for blue-white screening.
- Chemical transformation efficiency >108 cfu /μg DNA
- Genotype：endA1 recA1 gyrA96 thi-1 hsdR17 (rk-,mk+) relA1 supE44 D (lac-proAB) [F´ traD36 proAB laqI qZΔM15](DE3)
Cat. No. | Description | Packaging |
BL1863A | JM109 (DE3) Competent Cells | 10×100 μL |
