This strain is a specialized RNase III-deficient E. coli strain used for feeding nematodes and is primarily applied in RNA interference experiments with C. elegans. It grows normally in LB or 2YT medium, and the presence of the Tn10 transposon confers tetracycline resistance. The strain carries the λ phage DE3 region integrated into its chromosome (the DE3 region contains the T7 phage RNA polymerase gene, which can be induced by IPTG to express T7 RNA polymerase at high levels, initiating expression of double-stranded RNA for nematode experiments). It expresses both T7 RNA polymerase and E. coli RNA polymerase, making it suitable not only for RNAi experiments in nematodes but also for protein expression using pET, pGEX, and pMAL plasmids.
- Chemical transformation efficiency >0.5×108 cfu/μg DNA
- Genotype：F- mcrA mcrB,IN(rrnD-rrnE)1 rnc14::Tn10(DE3 lavUV5::T7 polymerase)
Cat. No. | Description | Packaging |
BL1861A | HT115 (DE3) Competent Cells | 10×100 μL |
This strain is a specialized RNase III-deficient E. coli strain used for feeding nematodes and is primarily applied in RNA interference experiments with C. elegans. It grows normally in LB or 2YT medium, and the presence of the Tn10 transposon confers tetracycline resistance. The strain carries the λ phage DE3 region integrated into its chromosome (the DE3 region contains the T7 phage RNA polymerase gene, which can be induced by IPTG to express T7 RNA polymerase at high levels, initiating expression of double-stranded RNA for nematode experiments). It expresses both T7 RNA polymerase and E. coli RNA polymerase, making it suitable not only for RNAi experiments in nematodes but also for protein expression using pET, pGEX, and pMAL plasmids.
- Chemical transformation efficiency >0.5×108 cfu/μg DNA
- Genotype：F- mcrA mcrB,IN(rrnD-rrnE)1 rnc14::Tn10(DE3 lavUV5::T7 polymerase)
Cat. No. | Description | Packaging |
BL1861A | HT115 (DE3) Competent Cells | 10×100 μL |
