E. coli contains the SbcCD protein complex, which recognizes and excises DNA hairpin structures. Mutations in the sbcC and sbcD genes enhance the stability of DNA hairpin structures. Additionally, introduction of *uidA(DMluI)::pir-116* into the E. coli genome enables GT115 to express the π protein, allowing normal replication of plasmids containing the R6Kγ ori replicon (such as pCpGmcs, pCpG-LacZ, pCpG-siRNA). This strain also carries mutations in endA (nuclease) and recA (recombinase), enhancing the stability of exogenous DNA.
- Chemical transformation efficiency >2×108 cfu/μg DNA
- Genotype：F- mcrAΔ(mrr-hsdRMS-mcrBC) f80lacZDM15ΔlacX74 recA1 rpsL (StrR) endA1 Δdcm uidA(DMluI)::pir-116ΔsbcC-sbcD
Cat. No. | Description | Packaging |
BL1859A | GT115 Competent Cells | 10×100 μL |
E. coli contains the SbcCD protein complex, which recognizes and excises DNA hairpin structures. Mutations in the sbcC and sbcD genes enhance the stability of DNA hairpin structures. Additionally, introduction of *uidA(DMluI)::pir-116* into the E. coli genome enables GT115 to express the π protein, allowing normal replication of plasmids containing the R6Kγ ori replicon (such as pCpGmcs, pCpG-LacZ, pCpG-siRNA). This strain also carries mutations in endA (nuclease) and recA (recombinase), enhancing the stability of exogenous DNA.
- Chemical transformation efficiency >2×108 cfu/μg DNA
- Genotype：F- mcrAΔ(mrr-hsdRMS-mcrBC) f80lacZDM15ΔlacX74 recA1 rpsL (StrR) endA1 Δdcm uidA(DMluI)::pir-116ΔsbcC-sbcD
Cat. No. | Description | Packaging |
BL1859A | GT115 Competent Cells | 10×100 μL |
