The EPI400 strain reduces plasmid copy number, making it particularly suitable for cloning unstable DNA or toxic genes. After the addition of WDEPI inducer I, plasmid yield can be restored to normal levels. The [mcrA, Δ(mrr-hsdRMS-mcrBC)] genotype makes the EPI400 strain suitable for cloning DNA rich in methylcytosine or methyladenine. Mutations in recA1 and endA1 facilitate stable insertion of DNA and high-purity plasmid DNA extraction. The presence of the lacZΔM15 marker enables blue-white screening in EPI400. tonA confers resistance to phages T1 and T5, and rpsL confers streptomycin resistance.
- Chemical transformation efficiency >5×107 cfu/μg DNA
- Genotype：F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA tonA pcnB4 dhfr
Cat. No. | Description | Packaging |
BL1858A | EPI400 Competent Cells | 10×100 μL |
The EPI400 strain reduces plasmid copy number, making it particularly suitable for cloning unstable DNA or toxic genes. After the addition of WDEPI inducer I, plasmid yield can be restored to normal levels. The [mcrA, Δ(mrr-hsdRMS-mcrBC)] genotype makes the EPI400 strain suitable for cloning DNA rich in methylcytosine or methyladenine. Mutations in recA1 and endA1 facilitate stable insertion of DNA and high-purity plasmid DNA extraction. The presence of the lacZΔM15 marker enables blue-white screening in EPI400. tonA confers resistance to phages T1 and T5, and rpsL confers streptomycin resistance.
- Chemical transformation efficiency >5×107 cfu/μg DNA
- Genotype：F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG trfA tonA pcnB4 dhfr
Cat. No. | Description | Packaging |
BL1858A | EPI400 Competent Cells | 10×100 μL |
