The EPI300 strain contains a mutated trfA gene, whose protein product promotes high-copy amplification of plasmids containing the oriV replicon. The inducer can induce expression of the trfA gene. The [mcrA, Δ(mrr-hsdRMS-mcrBC)] genotype makes the EPI300 strain suitable for cloning DNA rich in methylcytosine or methyladenine. Mutations in recA1 and endA1 facilitate stable insertion of DNA and high-purity plasmid DNA extraction. The presence of the lacZΔM15 marker enables blue-white screening in EPI300, and rpsL confers streptomycin resistance.
- Chemical transformation efficiency >3×108cfu/μg DNA
- Genotype：F‒ mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara,leu)7697 galU galK λ‒ rpsL nupG trfA dhfr
Cat. No. | Description | Packaging |
BL1857A | EPI300 Competent Cells | 10×100 μL |
The EPI300 strain contains a mutated trfA gene, whose protein product promotes high-copy amplification of plasmids containing the oriV replicon. The inducer can induce expression of the trfA gene. The [mcrA, Δ(mrr-hsdRMS-mcrBC)] genotype makes the EPI300 strain suitable for cloning DNA rich in methylcytosine or methyladenine. Mutations in recA1 and endA1 facilitate stable insertion of DNA and high-purity plasmid DNA extraction. The presence of the lacZΔM15 marker enables blue-white screening in EPI300, and rpsL confers streptomycin resistance.
- Chemical transformation efficiency >3×108cfu/μg DNA
- Genotype：F‒ mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara,leu)7697 galU galK λ‒ rpsL nupG trfA dhfr
Cat. No. | Description | Packaging |
BL1857A | EPI300 Competent Cells | 10×100 μL |
