The DH10Bac strain is primarily used for producing recombinant baculovirus molecules (Bac-to-Bac baculovirus expression system). The parental bacmid bMON14272 in this strain contains a mini-F replicon, a kanamycin resistance gene, an attTn7 site, and a lacZα complementation factor. The helper plasmid pMON7124 contains the tnsABCD region, confers tetracycline resistance, and may be lost during cell propagation but enhances transposition efficiency after transformation with the donor plasmid pFastBac (which confers gentamicin resistance). Mutations in mcrA, mcrBC, and mrr make the DH10Bac strain suitable for cloning DNA rich in methylcytosine or methyladenine. The recA1 and endA1 mutations facilitate stable insertion of DNA and high-purity plasmid DNA extraction. The presence of Φ80 lacZΔM15 enables blue-white screening in DH10Bac.
- Chemical transformation efficiency >108 cfu/μg DNA
- Genotype：F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ 80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG /pMON14272 / pMON7124
Cat. No. | Description | Packaging |
BL1855A | DH10Bac Competent Cells | 10×100 μL |
The DH10Bac strain is primarily used for producing recombinant baculovirus molecules (Bac-to-Bac baculovirus expression system). The parental bacmid bMON14272 in this strain contains a mini-F replicon, a kanamycin resistance gene, an attTn7 site, and a lacZα complementation factor. The helper plasmid pMON7124 contains the tnsABCD region, confers tetracycline resistance, and may be lost during cell propagation but enhances transposition efficiency after transformation with the donor plasmid pFastBac (which confers gentamicin resistance). Mutations in mcrA, mcrBC, and mrr make the DH10Bac strain suitable for cloning DNA rich in methylcytosine or methyladenine. The recA1 and endA1 mutations facilitate stable insertion of DNA and high-purity plasmid DNA extraction. The presence of Φ80 lacZΔM15 enables blue-white screening in DH10Bac.
- Chemical transformation efficiency >108 cfu/μg DNA
- Genotype：F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ 80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG /pMON14272 / pMON7124
Cat. No. | Description | Packaging |
BL1855A | DH10Bac Competent Cells | 10×100 μL |
