This strain is derived from MC1061. Mutations in mcrA, mcrBC, and mrr make DH10B suitable for cloning DNA rich in methylcytosine or methyladenine. The recA1 and endA1 mutations facilitate stable insertion of DNA and high-purity plasmid DNA extraction. The presence of Φ 80 lacZΔM15 enables blue-white screening in DH10B, and rpsL confers streptomycin resistance.
- Chemical transformation efficiency >108 cfu/μg DNA
- Genotype：F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ 80lacZΔM15ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galE15 galK λ-rpsL nupG
Cat. No. | Description | Packaging |
BL1854A | DH10B Competent Cells | 10×100 μL |
This strain is derived from MC1061. Mutations in mcrA, mcrBC, and mrr make DH10B suitable for cloning DNA rich in methylcytosine or methyladenine. The recA1 and endA1 mutations facilitate stable insertion of DNA and high-purity plasmid DNA extraction. The presence of Φ 80 lacZΔM15 enables blue-white screening in DH10B, and rpsL confers streptomycin resistance.
- Chemical transformation efficiency >108 cfu/μg DNA
- Genotype：F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ 80lacZΔM15ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galE15 galK λ-rpsL nupG
Cat. No. | Description | Packaging |
BL1854A | DH10B Competent Cells | 10×100 μL |
