BL21 is one of the earliest strains developed for prokaryotic expression. Strains such as BL21(DE3), Rosetta, and OrigamiB(DE3) are derived from BL21. This strain does not contain T7 RNA polymerase and cannot be used for protein expression driven by the T7 promoter (e.g., pET series plasmids). It contains E. coli RNA polymerase and is suitable for prokaryotic expression systems using E. coli RNA polymerase, such as tac or trc promoters (e.g., pGEX and pMAL plasmids). Transformation efficiency with pUC19 plasmid is > 10⁷ cfu/μg DNA.
- Chemical transformation efficiency >107 cfu/μg DNA
- Genotype：E. coli B F- dcm ompT hsdS(rB- mB-) gal [malB+]K-12(λS)
Cat. No. | Description | Packaging |
BL1848A | BL21 Competent Cells | 10×100 μL |
BL21 is one of the earliest strains developed for prokaryotic expression. Strains such as BL21(DE3), Rosetta, and OrigamiB(DE3) are derived from BL21. This strain does not contain T7 RNA polymerase and cannot be used for protein expression driven by the T7 promoter (e.g., pET series plasmids). It contains E. coli RNA polymerase and is suitable for prokaryotic expression systems using E. coli RNA polymerase, such as tac or trc promoters (e.g., pGEX and pMAL plasmids). Transformation efficiency with pUC19 plasmid is > 10⁷ cfu/μg DNA.
- Chemical transformation efficiency >107 cfu/μg DNA
- Genotype：E. coli B F- dcm ompT hsdS(rB- mB-) gal [malB+]K-12(λS)
Cat. No. | Description | Packaging |
BL1848A | BL21 Competent Cells | 10×100 μL |
