The TOP10 strain is derived from MC1061 and is one of the commonly used strains in laboratories, widely applied in molecular biology experiments such as gene cloning, blue-white screening, and stable plasmid amplification. Transformation efficiency with pUC19 plasmid can reach over 5 × 10⁸ cfu/μg DNA.
- Belongs to the *E. coli* K12 series, suitable for gene cloning, with fast growth rate
- For blue-white screening, only X-gal needs to be pre-coated on the plate; IPTG is not required
- Not suitable for protein expression using the T7 RNA polymerase expression system
- Free of exogenous plasmid DNA residue
- Chemical transformation efficiency: 5 × 10⁸ cfu/μg pUC19 DNA
- Genotype: F⁻ mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG
Cat. No. | Description | Packaging |
BL1289A | TOP10 Competent Cells | 25×100μL |
BL1289B | TOP10 Competent Cells | 100×100μL |
The TOP10 strain is derived from MC1061 and is one of the commonly used strains in laboratories, widely applied in molecular biology experiments such as gene cloning, blue-white screening, and stable plasmid amplification. Transformation efficiency with pUC19 plasmid can reach over 5 × 10⁸ cfu/μg DNA.
- Belongs to the *E. coli* K12 series, suitable for gene cloning, with fast growth rate
- For blue-white screening, only X-gal needs to be pre-coated on the plate; IPTG is not required
- Not suitable for protein expression using the T7 RNA polymerase expression system
- Free of exogenous plasmid DNA residue
- Chemical transformation efficiency: 5 × 10⁸ cfu/μg pUC19 DNA
- Genotype: F⁻ mcrA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG
Cat. No. | Description | Packaging |
BL1289A | TOP10 Competent Cells | 25×100μL |
BL1289B | TOP10 Competent Cells | 100×100μL |
