This product is a high-efficiency chemically competent cell prepared from E. coli strain BL21(DE3), which contains the T7 RNA polymerase gene (λDE3) under the control of the lacUV5 promoter, enabling IPTG-induced expression of T7 RNA polymerase. Transformation efficiency exceeds 10⁶ cfu/μg DNA, making it suitable for high-efficiency protein expression using pET series plasmids.
- BL21(DE3) strain, T7 RNA polymerase gene located in the λ phage DE3 region integrated into the BL21 chromosome
- High-efficiency protein expression host utilizing the T7 RNA polymerase expression system, suitable for recombinant expression of non-viral proteins
- Free of exogenous plasmid DNA residue
- Chemical transformation efficiency ≥ 10⁶ cfu/μg pUC19 DNA
- Genotype: F⁻ ompT hsdSB (rB⁻ mB⁻) gal dcm (DE3)
Cat. No. | Description | Packaging |
BL1287A | BL21(DE3) Competent Cells | 25×100μL |
BL1287B | BL21(DE3) Competent Cells | 100×100μL |
This product is a high-efficiency chemically competent cell prepared from E. coli strain BL21(DE3), which contains the T7 RNA polymerase gene (λDE3) under the control of the lacUV5 promoter, enabling IPTG-induced expression of T7 RNA polymerase. Transformation efficiency exceeds 10⁶ cfu/μg DNA, making it suitable for high-efficiency protein expression using pET series plasmids.
- BL21(DE3) strain, T7 RNA polymerase gene located in the λ phage DE3 region integrated into the BL21 chromosome
- High-efficiency protein expression host utilizing the T7 RNA polymerase expression system, suitable for recombinant expression of non-viral proteins
- Free of exogenous plasmid DNA residue
- Chemical transformation efficiency ≥ 10⁶ cfu/μg pUC19 DNA
- Genotype: F⁻ ompT hsdSB (rB⁻ mB⁻) gal dcm (DE3)
Cat. No. | Description | Packaging |
BL1287A | BL21(DE3) Competent Cells | 25×100μL |
BL1287B | BL21(DE3) Competent Cells | 100×100μL |
