Molecular cloning provides a method for purifying and amplifying specific DNA fragments at the molecular level. The target gene is often inserted into a cloning vector through in vitro recombination to form a recombinant cloning vector. This vector is then introduced into a suitable host via transformation or transduction for replication and amplification. Subsequently, the desired cloning vector is isolated and purified from the selected host cells, yielding multiple copies of the inserted DNA and achieving amplification of the target gene.
GUS Staining Kit
The GUS (β-glucuronidase) gene is a commonly used reporter gene. Its expression product, β-glucuronidase (GUS), is a hydrolase that catalyzes the hydrolysis of various β-glucuronides. It can cleave 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-Gluc) into a blue product. The detection method is simple, rapid, sensitive, stable, and exhibits low background activity. Since most plant cells lack endogenous GUS activity, the gus gene is widely used as a reporter gene in transgenic plants, particularly in transformation experiments studying transient expression of foreign genes.
Cat. No. | Description | Packaging |
BL622A | GUS Staining Kit | 50mL |
One-Step Cloning Kit
This kit is an efficient and rapid in vitro recombination kit designed based on homologous recombination and the self-repair function of *E. coli*, eliminating the need for restriction digestion and ligation.
- Simple operation, time-saving - No need to consider restriction sites of vector or sequence; allows insertion of target sequence into any position of the vector - Capable of simultaneous ligation of multiple fragments (1‒5) - User-friendly packaging for convenient operation, ensuring enzyme activity - Significantly reduces experimental cost while maintaining quality |  | |||||
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Other Molecular Cloning Related Products
Cat. No. | Description | Packaging |
BL545A | IPTG Solution (50 mg/mL) | 5mL |
BL546A | X-gal Solution (20 mg/mL) | 5mL |
BL610A | Ampicillin Solution | 5mL |
BL611A | Kanamycin Solution | 5×1mL |
BL1178A | Calcium Chloride Solution (1 mol/L, sterile) | 100mL |
TOPO Cloning Kit
TOPO cloning utilizes the ligation mechanism of topoisomerase I, enabling efficient ligation of DNA fragments in an instant (within minutes or even seconds). The kit contains a vector with a suicide gene. When the vector is successfully ligated with the insert, the suicide gene is not correctly expressed, allowing cells containing the recombinant to grow normally. When ligation fails, the suicide gene is correctly expressed, preventing growth of cells containing the vector, resulting in zero background.
Cat. No. | Description | Packaging |
BL1326A | TA/Blunt Zero Background pTOPO Cloning Kit | 20rxn |
BL1326B | TA/Blunt Zero Background pTOPO Cloning Kit | 100rxn |
Molecular cloning provides a method for purifying and amplifying specific DNA fragments at the molecular level. The target gene is often inserted into a cloning vector through in vitro recombination to form a recombinant cloning vector. This vector is then introduced into a suitable host via transformation or transduction for replication and amplification. Subsequently, the desired cloning vector is isolated and purified from the selected host cells, yielding multiple copies of the inserted DNA and achieving amplification of the target gene.
GUS Staining Kit
The GUS (β-glucuronidase) gene is a commonly used reporter gene. Its expression product, β-glucuronidase (GUS), is a hydrolase that catalyzes the hydrolysis of various β-glucuronides. It can cleave 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-Gluc) into a blue product. The detection method is simple, rapid, sensitive, stable, and exhibits low background activity. Since most plant cells lack endogenous GUS activity, the gus gene is widely used as a reporter gene in transgenic plants, particularly in transformation experiments studying transient expression of foreign genes.
Cat. No. | Description | Packaging |
BL622A | GUS Staining Kit | 50mL |
One-Step Cloning Kit
This kit is an efficient and rapid in vitro recombination kit designed based on homologous recombination and the self-repair function of *E. coli*, eliminating the need for restriction digestion and ligation.
- Simple operation, time-saving - No need to consider restriction sites of vector or sequence; allows insertion of target sequence into any position of the vector - Capable of simultaneous ligation of multiple fragments (1‒5) - User-friendly packaging for convenient operation, ensuring enzyme activity - Significantly reduces experimental cost while maintaining quality | | |||||
|
Other Molecular Cloning Related Products
Cat. No. | Description | Packaging |
BL545A | IPTG Solution (50 mg/mL) | 5mL |
BL546A | X-gal Solution (20 mg/mL) | 5mL |
BL610A | Ampicillin Solution | 5mL |
BL611A | Kanamycin Solution | 5×1mL |
BL1178A | Calcium Chloride Solution (1 mol/L, sterile) | 100mL |
TOPO Cloning Kit
TOPO cloning utilizes the ligation mechanism of topoisomerase I, enabling efficient ligation of DNA fragments in an instant (within minutes or even seconds). The kit contains a vector with a suicide gene. When the vector is successfully ligated with the insert, the suicide gene is not correctly expressed, allowing cells containing the recombinant to grow normally. When ligation fails, the suicide gene is correctly expressed, preventing growth of cells containing the vector, resulting in zero background.
Cat. No. | Description | Packaging |
BL1326A | TA/Blunt Zero Background pTOPO Cloning Kit | 20rxn |
BL1326B | TA/Blunt Zero Background pTOPO Cloning Kit | 100rxn |
