Nucleic acid electrophoresis is an essential technique in nucleic acid research and a critical component of methods such as nucleic acid probes, amplification, and sequence analysis. It is typically performed in agarose or polyacrylamide gels. Agarose and polyacrylamide at different concentrations form gels with varying pore sizes, allowing separation of nucleic acid fragments of different molecular weights.
Nucleic Acid Electrophoresis
Nucleic acid stains are indispensable in biological experiments, particularly in nucleic acid detection. Their mechanism involves electrostatic adsorption to DNA molecules, emitting specific fluorescence upon light exposure, significantly enhancing DNA visualization. Our company offers a comprehensive range of mainstream nucleic acid stains, providing diverse options to meet various needs.
Comparison of Different Nucleic Acid Stains
Cat. No. | Description | Packaging |
BS353B | Nucleic Acid Stain Pure Red | 500μL |
Electrophoresis Buffer
Nucleic acid electrophoresis buffers are commonly used saline solutions in molecular biology, primarily for DNA agarose gel electrophoresis. TBE buffer, composed of Tris-borate and EDTA, offers strong buffering capacity, making it suitable for prolonged electrophoresis and providing high resolution, especially for fragments smaller than 1 kb. However, the borate in TBE can affect DNA recovery efficiency and subsequent enzymatic reactions. For DNA fragment recovery from agarose gels, TAE buffer is recommended.
Cat. No. | Description | Packaging |
BL533A | TAE (50×) Solution | 500mL |
BL540A | 10× TBE Solution | 250mL |
BL540B | 10× TBE Solution | 500mL |
BL548A | 5× TBE Solution | 500mL |
BL1285A | TAE Instant Powder | 10 × 1L |
BL1286A | TBE Instant Powder | 10 × 1L |
Loading Buffer
Nucleic acid loading buffer is a suitably concentrated loading buffer, primarily using bromophenol blue or other dyes as indicators. When diluted to 1×, it maintains a high density, allowing easy sinking during loading with clear color visibility. Nucleic acid loading buffers can be classified into RNA and DNA loading buffers.
Cat. No. | Description | Packaging |
BL783A | 6× DNA Loading Buffer (Red) | 2×1mL |
Nucleic acid electrophoresis is an essential technique in nucleic acid research and a critical component of methods such as nucleic acid probes, amplification, and sequence analysis. It is typically performed in agarose or polyacrylamide gels. Agarose and polyacrylamide at different concentrations form gels with varying pore sizes, allowing separation of nucleic acid fragments of different molecular weights.
Nucleic Acid Electrophoresis
Nucleic acid stains are indispensable in biological experiments, particularly in nucleic acid detection. Their mechanism involves electrostatic adsorption to DNA molecules, emitting specific fluorescence upon light exposure, significantly enhancing DNA visualization. Our company offers a comprehensive range of mainstream nucleic acid stains, providing diverse options to meet various needs.
Comparison of Different Nucleic Acid Stains
Cat. No. | Description | Packaging |
BS353B | Nucleic Acid Stain Pure Red | 500μL |
Electrophoresis Buffer
Nucleic acid electrophoresis buffers are commonly used saline solutions in molecular biology, primarily for DNA agarose gel electrophoresis. TBE buffer, composed of Tris-borate and EDTA, offers strong buffering capacity, making it suitable for prolonged electrophoresis and providing high resolution, especially for fragments smaller than 1 kb. However, the borate in TBE can affect DNA recovery efficiency and subsequent enzymatic reactions. For DNA fragment recovery from agarose gels, TAE buffer is recommended.
Cat. No. | Description | Packaging |
BL533A | TAE (50×) Solution | 500mL |
BL540A | 10× TBE Solution | 250mL |
BL540B | 10× TBE Solution | 500mL |
BL548A | 5× TBE Solution | 500mL |
BL1285A | TAE Instant Powder | 10 × 1L |
BL1286A | TBE Instant Powder | 10 × 1L |
Loading Buffer
Nucleic acid loading buffer is a suitably concentrated loading buffer, primarily using bromophenol blue or other dyes as indicators. When diluted to 1×, it maintains a high density, allowing easy sinking during loading with clear color visibility. Nucleic acid loading buffers can be classified into RNA and DNA loading buffers.
Cat. No. | Description | Packaging |
BL783A | 6× DNA Loading Buffer (Red) | 2×1mL |
