Nucleic acid electrophoresis is an essential technique in nucleic acid research and a critical component of methods such as nucleic acid probes, amplification, and sequence analysis. It is typically performed in agarose or polyacrylamide gels. Agarose and polyacrylamide at different concentrations form gels with varying pore sizes, allowing separation of nucleic acid fragments of different molecular weights.
Nucleic Acid Electrophoresis
Nucleic acid stains are indispensable in biological experiments, particularly in nucleic acid detection. Their mechanism involves electrostatic adsorption to DNA molecules, emitting specific fluorescence upon light exposure, significantly enhancing DNA visualization. Our company offers a comprehensive range of mainstream nucleic acid stains, providing diverse options to meet various needs.
Comparison of Different Nucleic Acid Stains
Cat. No. | Description | Packaging |
BS353B | Nucleic Acid Stain Pure Red | 500μL |
BS354A | Nucleic Acid Stain Super Red | 100μL |
BS354B | Nucleic Acid Stain Super Red | 500μL |
BS355A | Nucleic Acid Stain Super Green | 500μL |
BS356A | Nucleic Acid Stain Safe Green | 500μL |
BS357A | Nucleic Acid Stain GoldView | 1mL |
BS358A | Nucleic Acid Stain SYBR Green I in DMSO | 100μL |
BS359A | Nucleic Acid Stain SYBR Green II in DMSO | 100μL |
BL1672A | Nucleic Acid Stain Safe Gold | 500μL |
Electrophoresis Buffer
Nucleic acid electrophoresis buffers are commonly used saline solutions in molecular biology, primarily for DNA agarose gel electrophoresis. TBE buffer, composed of Tris-borate and EDTA, offers strong buffering capacity, making it suitable for prolonged electrophoresis and providing high resolution, especially for fragments smaller than 1 kb. However, the borate in TBE can affect DNA recovery efficiency and subsequent enzymatic reactions. For DNA fragment recovery from agarose gels, TAE buffer is recommended.
Cat. No. | Description | Packaging |
BL533A | TAE (50×) Solution | 500mL |
BL540A | 10× TBE Solution | 250mL |
BL540B | 10× TBE Solution | 500mL |
BL548A | 5× TBE Solution | 500mL |
BL1285A | TAE Instant Powder | 10 × 1L |
BL1286A | TBE Instant Powder | 10 × 1L |
Loading Buffer
Nucleic acid loading buffer is a suitably concentrated loading buffer, primarily using bromophenol blue or other dyes as indicators. When diluted to 1×, it maintains a high density, allowing easy sinking during loading with clear color visibility. Nucleic acid loading buffers can be classified into RNA and DNA loading buffers.
Cat. No. | Description | Packaging |
BL544A | 5× RNA Loading Buffer | 1mL |
BL532A | DNA Loading Buffer (6×) | 2×1mL |
BL783A | 6× DNA Loading Buffer (Red) | 2×1mL |
BL1575A | 2× Urea-TBE Loading Buffer | 1mL |
Precast Agarose Gel
Biosharp precast agarose gel is a high-performance gel suitable for nucleic acid electrophoresis detection (RNase-free version recommended for RNA electrophoresis), providing clear and straight bands. The product features high-capacity wells for easy sample loading, with nucleic acid dyes pre-mixed in the gel, compatible with various gel imaging systems (UV, blue light, etc.). Samples can be directly loaded after adding loading buffer, allowing direct electrophoresis and band visualization.
Cat. No. | Description | Packaging |
BL1149A | Precast Agarose Gel (1%, TBE, 8 wells) | 10 sheets/box |
BL1150A | Precast Agarose Gel (1%, TBE, 8 wells, RNase-free) | 10 sheets/box |
BL1151A | Precast Agarose Gel (2%, TBE, 8 wells) | 10 sheets/box |
BL1152A | Precast Agarose Gel (2%, TBE, 8 wells, RNase-free) | 10 sheets/box |
BL1153A | Precast Agarose Gel (3%, TBE, 8 wells) | 10 sheets/box |
BL1154A | Precast Agarose Gel (3%, TBE, 8 wells, RNase-free) | 10 sheets/box |
BL1155A | Precast Agarose Gel (1%, TBE, 15 wells) | 5 sheets/box |
BL1156A | Precast Agarose Gel (2%, TBE, 15 wells) | 5 sheets/box |
BL1157A | Precast Agarose Gel (3%, TBE, 15 wells) | 5 sheets/box |
BL1158A | Precast Agarose Gel (1%, TAE, 8 wells) | 10 sheets/box |
BL1159A | Precast Agarose Gel (1%, TAE, 8 wells, RNase-free) | 10 sheets/box |
BL1160A | Precast Agarose Gel (2%, TAE, 8 wells) | 10 sheets/box |
BL1161A | Precast Agarose Gel (2%, TAE, 8 wells, RNase-free) | 10 sheets/box |
BL1162A | Precast Agarose Gel (3%, TAE, 8 wells) | 10 sheets/box |
BL1163A | Precast Agarose Gel (3%, TAE, 8 wells, RNase-free) | 10 sheets/box |
BL1164A | Precast Agarose Gel (1%, TAE, 15 wells) | 5 sheets/box |
BL1165A | Precast Agarose Gel (2%, TAE, 15 wells) | 5 sheets/box |
BL1166A | Precast Agarose Gel (3%, TAE, 15 wells) | 5 sheets/box |
BL1494A | Denaturing Nucleic Acid Precast Gel, 5%, 10 wells, 1.5 mm | 10 sheets/box |
BL1495A | Denaturing Nucleic Acid Precast Gel, 5%, 15 wells, 1.5 mm | 10 sheets/box |
BL1496A | Denaturing Nucleic Acid Precast Gel, 10%, 10 wells, 1.5 mm | 10 sheets/box |
BL1497A | Denaturing Nucleic Acid Precast Gel, 10%, 15 wells, 1.5 mm | 10 sheets/box |
BL1498A | Denaturing Nucleic Acid Precast Gel, 15%, 10 wells, 1.5 mm | 10 sheets/box |
BL1499A | Denaturing Nucleic Acid Precast Gel, 15%, 15 wells, 1.5 mm | 10 sheets/box |
BL1500A | Denaturing Nucleic Acid Precast Gel, 20%, 10 wells, 1.5 mm | 10 sheets/box |
BL1501A | Denaturing Nucleic Acid Precast Gel, 20%, 15 wells, 1.5 mm | 10 sheets/box |
BL1502A | Non-Denaturing Nucleic Acid Precast Gel, 5%, 10 wells, 1.5 mm | 10 sheets/box |
BL1503A | Non-Denaturing Nucleic Acid Precast Gel, 5%, 15 wells, 1.5 mm | 10 sheets/box |
BL1504A | Non-Denaturing Nucleic Acid Precast Gel, 10%, 10 wells, 1.5 mm | 10 sheets/box |
BL1505A | Non-Denaturing Nucleic Acid Precast Gel, 10%, 15 wells, 1.5 mm | 10 sheets/box |
BL1506A | Non-Denaturing Nucleic Acid Precast Gel, 12%, 10 wells, 1.5 mm | 10 sheets/box |
BL1507A | Non-Denaturing Nucleic Acid Precast Gel, 12%, 15 wells, 1.5 mm | 10 sheets/box |
BL1508A | Non-Denaturing Nucleic Acid Precast Gel, 15%, 10 wells, 1.5 mm | 10 sheets/box |
BL1509A | Non-Denaturing Nucleic Acid Precast Gel, 15%, 15 wells, 1.5 mm | 10 sheets/box |
BL1510A | Non-Denaturing Nucleic Acid Precast Gel, 4-20%, 10 wells, 1.5 mm | 10 sheets/box |
BL1511A | Non-Denaturing Nucleic Acid Precast Gel, 4-20%, 15 wells, 1.5 mm | 10 sheets/box |
EMSA Precast Gel
Electrophoretic mobility shift assay (EMSA), also known as gel shift assay or gel retardation assay, is a technique used to study
protein-nucleic acid interactions. Initially developed for validating transcription factor-promoter interactions, it is also applicable to
protein-DNA and protein-RNA interaction studies.
Cat. No. | Description | Packaging |
BL1488A | EMSA Precast Gel, 4%, 10 wells, 1.5 mm | 10 sheets/box |
BL1489A | EMSA Precast Gel, 4%, 15 wells, 1.5 mm | 10 sheets/box |
BL1490A | EMSA Precast Gel, 6%, 10 wells, 1.5 mm | 10 sheets/box |
BL1491A | EMSA Precast Gel, 6%, 15 wells, 1.5 mm | 10 sheets/box |
EMSA Loading Buffer
EMSA can be used to study the binding between a target protein and a specific DNA sequence, thereby assessing the activation levels
of intracellular transcription factors. EMSA/Gel-Shift Loading Buffer is used for loading samples after the binding reaction between
nuclear proteins or purified transcription factors and specific double-stranded oligonucleotides in EMSA studies.
4× EMSA/Gel-Shift Loading Buffer (Blue) contains bromophenol blue, which may sometimes interfere with the binding reaction
between the target transcription factor and the specific double-stranded oligonucleotide. If interference occurs, 4× EMSA/Gel-Shift
Loading Buffer (Colorless) without bromophenol blue can be used.
Cat. No. | Description | Packaging |
BL1576A | 4× EMSA/Gel-Shift Loading Buffer (Colorless) | 1mL |
BL1577A | 4× EMSA/Gel-Shift Loading Buffer (Blue) | 1mL |
Nucleic acid electrophoresis is an essential technique in nucleic acid research and a critical component of methods such as nucleic acid probes, amplification, and sequence analysis. It is typically performed in agarose or polyacrylamide gels. Agarose and polyacrylamide at different concentrations form gels with varying pore sizes, allowing separation of nucleic acid fragments of different molecular weights.
Nucleic Acid Electrophoresis
Nucleic acid stains are indispensable in biological experiments, particularly in nucleic acid detection. Their mechanism involves electrostatic adsorption to DNA molecules, emitting specific fluorescence upon light exposure, significantly enhancing DNA visualization. Our company offers a comprehensive range of mainstream nucleic acid stains, providing diverse options to meet various needs.
Comparison of Different Nucleic Acid Stains
Cat. No. | Description | Packaging |
BS353B | Nucleic Acid Stain Pure Red | 500μL |
BS354A | Nucleic Acid Stain Super Red | 100μL |
BS354B | Nucleic Acid Stain Super Red | 500μL |
BS355A | Nucleic Acid Stain Super Green | 500μL |
BS356A | Nucleic Acid Stain Safe Green | 500μL |
BS357A | Nucleic Acid Stain GoldView | 1mL |
BS358A | Nucleic Acid Stain SYBR Green I in DMSO | 100μL |
BS359A | Nucleic Acid Stain SYBR Green II in DMSO | 100μL |
BL1672A | Nucleic Acid Stain Safe Gold | 500μL |
Electrophoresis Buffer
Nucleic acid electrophoresis buffers are commonly used saline solutions in molecular biology, primarily for DNA agarose gel electrophoresis. TBE buffer, composed of Tris-borate and EDTA, offers strong buffering capacity, making it suitable for prolonged electrophoresis and providing high resolution, especially for fragments smaller than 1 kb. However, the borate in TBE can affect DNA recovery efficiency and subsequent enzymatic reactions. For DNA fragment recovery from agarose gels, TAE buffer is recommended.
Cat. No. | Description | Packaging |
BL533A | TAE (50×) Solution | 500mL |
BL540A | 10× TBE Solution | 250mL |
BL540B | 10× TBE Solution | 500mL |
BL548A | 5× TBE Solution | 500mL |
BL1285A | TAE Instant Powder | 10 × 1L |
BL1286A | TBE Instant Powder | 10 × 1L |
Loading Buffer
Nucleic acid loading buffer is a suitably concentrated loading buffer, primarily using bromophenol blue or other dyes as indicators. When diluted to 1×, it maintains a high density, allowing easy sinking during loading with clear color visibility. Nucleic acid loading buffers can be classified into RNA and DNA loading buffers.
Cat. No. | Description | Packaging |
BL544A | 5× RNA Loading Buffer | 1mL |
BL532A | DNA Loading Buffer (6×) | 2×1mL |
BL783A | 6× DNA Loading Buffer (Red) | 2×1mL |
BL1575A | 2× Urea-TBE Loading Buffer | 1mL |
Precast Agarose Gel
Biosharp precast agarose gel is a high-performance gel suitable for nucleic acid electrophoresis detection (RNase-free version recommended for RNA electrophoresis), providing clear and straight bands. The product features high-capacity wells for easy sample loading, with nucleic acid dyes pre-mixed in the gel, compatible with various gel imaging systems (UV, blue light, etc.). Samples can be directly loaded after adding loading buffer, allowing direct electrophoresis and band visualization.
Cat. No. | Description | Packaging |
BL1149A | Precast Agarose Gel (1%, TBE, 8 wells) | 10 sheets/box |
BL1150A | Precast Agarose Gel (1%, TBE, 8 wells, RNase-free) | 10 sheets/box |
BL1151A | Precast Agarose Gel (2%, TBE, 8 wells) | 10 sheets/box |
BL1152A | Precast Agarose Gel (2%, TBE, 8 wells, RNase-free) | 10 sheets/box |
BL1153A | Precast Agarose Gel (3%, TBE, 8 wells) | 10 sheets/box |
BL1154A | Precast Agarose Gel (3%, TBE, 8 wells, RNase-free) | 10 sheets/box |
BL1155A | Precast Agarose Gel (1%, TBE, 15 wells) | 5 sheets/box |
BL1156A | Precast Agarose Gel (2%, TBE, 15 wells) | 5 sheets/box |
BL1157A | Precast Agarose Gel (3%, TBE, 15 wells) | 5 sheets/box |
BL1158A | Precast Agarose Gel (1%, TAE, 8 wells) | 10 sheets/box |
BL1159A | Precast Agarose Gel (1%, TAE, 8 wells, RNase-free) | 10 sheets/box |
BL1160A | Precast Agarose Gel (2%, TAE, 8 wells) | 10 sheets/box |
BL1161A | Precast Agarose Gel (2%, TAE, 8 wells, RNase-free) | 10 sheets/box |
BL1162A | Precast Agarose Gel (3%, TAE, 8 wells) | 10 sheets/box |
BL1163A | Precast Agarose Gel (3%, TAE, 8 wells, RNase-free) | 10 sheets/box |
BL1164A | Precast Agarose Gel (1%, TAE, 15 wells) | 5 sheets/box |
BL1165A | Precast Agarose Gel (2%, TAE, 15 wells) | 5 sheets/box |
BL1166A | Precast Agarose Gel (3%, TAE, 15 wells) | 5 sheets/box |
BL1494A | Denaturing Nucleic Acid Precast Gel, 5%, 10 wells, 1.5 mm | 10 sheets/box |
BL1495A | Denaturing Nucleic Acid Precast Gel, 5%, 15 wells, 1.5 mm | 10 sheets/box |
BL1496A | Denaturing Nucleic Acid Precast Gel, 10%, 10 wells, 1.5 mm | 10 sheets/box |
BL1497A | Denaturing Nucleic Acid Precast Gel, 10%, 15 wells, 1.5 mm | 10 sheets/box |
BL1498A | Denaturing Nucleic Acid Precast Gel, 15%, 10 wells, 1.5 mm | 10 sheets/box |
BL1499A | Denaturing Nucleic Acid Precast Gel, 15%, 15 wells, 1.5 mm | 10 sheets/box |
BL1500A | Denaturing Nucleic Acid Precast Gel, 20%, 10 wells, 1.5 mm | 10 sheets/box |
BL1501A | Denaturing Nucleic Acid Precast Gel, 20%, 15 wells, 1.5 mm | 10 sheets/box |
BL1502A | Non-Denaturing Nucleic Acid Precast Gel, 5%, 10 wells, 1.5 mm | 10 sheets/box |
BL1503A | Non-Denaturing Nucleic Acid Precast Gel, 5%, 15 wells, 1.5 mm | 10 sheets/box |
BL1504A | Non-Denaturing Nucleic Acid Precast Gel, 10%, 10 wells, 1.5 mm | 10 sheets/box |
BL1505A | Non-Denaturing Nucleic Acid Precast Gel, 10%, 15 wells, 1.5 mm | 10 sheets/box |
BL1506A | Non-Denaturing Nucleic Acid Precast Gel, 12%, 10 wells, 1.5 mm | 10 sheets/box |
BL1507A | Non-Denaturing Nucleic Acid Precast Gel, 12%, 15 wells, 1.5 mm | 10 sheets/box |
BL1508A | Non-Denaturing Nucleic Acid Precast Gel, 15%, 10 wells, 1.5 mm | 10 sheets/box |
BL1509A | Non-Denaturing Nucleic Acid Precast Gel, 15%, 15 wells, 1.5 mm | 10 sheets/box |
BL1510A | Non-Denaturing Nucleic Acid Precast Gel, 4-20%, 10 wells, 1.5 mm | 10 sheets/box |
BL1511A | Non-Denaturing Nucleic Acid Precast Gel, 4-20%, 15 wells, 1.5 mm | 10 sheets/box |
EMSA Precast Gel
Electrophoretic mobility shift assay (EMSA), also known as gel shift assay or gel retardation assay, is a technique used to study
protein-nucleic acid interactions. Initially developed for validating transcription factor-promoter interactions, it is also applicable to
protein-DNA and protein-RNA interaction studies.
Cat. No. | Description | Packaging |
BL1488A | EMSA Precast Gel, 4%, 10 wells, 1.5 mm | 10 sheets/box |
BL1489A | EMSA Precast Gel, 4%, 15 wells, 1.5 mm | 10 sheets/box |
BL1490A | EMSA Precast Gel, 6%, 10 wells, 1.5 mm | 10 sheets/box |
BL1491A | EMSA Precast Gel, 6%, 15 wells, 1.5 mm | 10 sheets/box |
EMSA Loading Buffer
EMSA can be used to study the binding between a target protein and a specific DNA sequence, thereby assessing the activation levels
of intracellular transcription factors. EMSA/Gel-Shift Loading Buffer is used for loading samples after the binding reaction between
nuclear proteins or purified transcription factors and specific double-stranded oligonucleotides in EMSA studies.
4× EMSA/Gel-Shift Loading Buffer (Blue) contains bromophenol blue, which may sometimes interfere with the binding reaction
between the target transcription factor and the specific double-stranded oligonucleotide. If interference occurs, 4× EMSA/Gel-Shift
Loading Buffer (Colorless) without bromophenol blue can be used.
Cat. No. | Description | Packaging |
BL1576A | 4× EMSA/Gel-Shift Loading Buffer (Colorless) | 1mL |
BL1577A | 4× EMSA/Gel-Shift Loading Buffer (Blue) | 1mL |
