PCR (Polymerase Chain Reaction) is a technique for in vitro amplification of specific DNA sequences. The principle involves the replication of complementary DNA strands from a template DNA using DNA polymerase and specific primers through cycles of denaturation, annealing, and extension. It enables rapid and specific amplification of target DNA and is widely used in gene isolation and cloning, sequence analysis, gene expression regulation, and polymorphism studies.
Taq plus PCR Mix
- High efficiency: Amplifies up to 8 kb with λDNA template, efficiently amplifies fragments ≤4 kb - High sensitivity: Amplifies specific gene fragments from 0.05 ng human genomic DNA template - Stable: Maintains amplification performance after repeated freeze-thaw cycles, 30 days at 4°C, or one week at room temperature - Fast: All necessary reagents are provided in a single tube, allowing reaction setup within minutes - Convenient: Direct electrophoresis after PCR reaction |
Experimental Data
A. Amplification detection of mouse tail samples.
Samples 1‒5 represent different brands, with B1 as BL553A and B2 as BL547A. Both enzymes produced clear bands and high amplification yields with different primer sets.
B. Amplification detection of rice samples.
P1‒P7 represent amplification primers for different product sizes. Amplification was stable, with clear bands and high yields across all primers.
Cat. No. | Description | Packaging |
BL553A | 2× Taq Plus PCR Master Mix with Dye | 1mL |
BL553B | 2× Taq Plus PCR Master Mix with Dye | 1mL×5 |
BL553C | 2× Taq Plus PCR Master Mix with Dye | 1mL×25 |
BL553D | 2× Taq Plus PCR Master Mix with Dye | 1mL×100 |
BL1361A | 2× Taq Plus PCR Master Mix without Dye | 1mL |
BL1361B | 2× Taq Plus PCR Master Mix without Dye | 5×1mL |
BL1361C | 2× Taq Plus PCR Master Mix without Dye | 25×1mL |
BL1361D | 2× Taq Plus PCR Master Mix without Dye | 100×1mL |
Fast Taq plus Master Mix
- Faster amplification: 5s/kb for routine templates below 3kb, up to 1s/kb; PCR completed within 30 minutes - Wider applicability: Suitable for templates with high GC content and complex secondary structures - Superior performance: High specificity and yield - Easier operation: Ready-to-use premix, optional red dye for easy identification |
Cat. No. | Description | Packaging |
BL971A | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification) | 1mL |
BL971B | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification) | 10×1mL |
BL971C | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification) | 100×1mL |
BL1014A | 2× Fast Taq Plus PCR Master Mix without Dye (for Rapid Amplification) | 1mL |
BL1014B | 2× Fast Taq Plus PCR Master Mix without Dye (for Rapid Amplification) | 10×1mL |
BL1014C | 2× Fast Taq Plus PCR Master Mix without Dye (for Rapid Amplification) | 100×1mL |
BL1513A | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification, Low Background) | 1mL |
BL1513B | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification, Low Background) | 10×1mL |
BL1513C | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification, Low Background) | 100×1mL |
Pfu PCR Mix
- Only requires the addition of primers and template for PCR, minimizing human error, with fast and simple operation and good stability - Used for high-fidelity amplification of DNA fragments - Amplification products are blunt-ended, suitable for blunt-end cloning |
Cat. No. | Description | Packaging |
BL111A | 2× Pfu PCR Master Mix with Dye | 1mL |
Quality Assurance
- Amplifies a 5 kb DNA fragment efficiently using λ DNA as a template
- Amplifies a 3.2 kb DNA fragment efficiently using rice genomic DNA as a template
Taq plus PCR Mix for PAGE
- Direct loading: PCR products with dye can be directly loaded for gel electrophoresis - High sensitivity: High-purity enzyme ensures excellent sensitivity - No genomic contamination: Amplified products are of high purity - Efficient amplification: Optimized buffer system enhances amplification performance - Good reproducibility: Premixed system reduces pipetting errors and contamination risk - Batch-to-batch consistency: Standardized production process and strict quality control |
Cat. No. | Description | Packaging |
BL631A | 2× Taq PCR Master Mix for PAGE with Dye | 1mL |
BL631B | 2× Taq PCR Master Mix for PAGE with Dye | 5×1mL |
BL634A | 50 bp DNA Marker (50‒600 bp) | 1mL |
BL1514A | 2× Super Taq PCR Master Mix for PAGE with Dye (for Rapid Amplification) | 1mL |
BL1514B | 2× Super Taq PCR Master Mix for PAGE with Dye (for Rapid Amplification) | 5×1mL |
Super Taq PCR Premix
- Possesses 5′→3′ DNA polymerase activity and 3′→5′ exonuclease activity - 6-fold higher fidelity than standard Taq DNA Polymerase, with high sensitivity and good stability - Strong synthesis capability, suitable for amplification of complex templates and rapid amplification - Pre-mixed PCR format minimizes human error, saves time, and reduces contamination risk - PCR products have a 3′-terminal overhang "A" base, allowing direct cloning into T-vectors |
Cat. No. | Description | Packaging |
BL1015A | 2× Super Taq Plus PCR Master Mix with Dye (for Complex/High GC Template Amplification) | 1mL |
BL1015B | 2× Super Taq Plus PCR Master Mix with Dye (for Complex/High GC Template Amplification) | 5×1mL |
BL1015C | 2× Super Taq Plus PCR Master Mix with Dye (for Complex/High GC Template Amplification) | 10×1mL |
BL1309A | 2× Super Taq Plus PCR Master Mix without Dye (for Complex/High GC Template Amplification) | 1mL |
BL5824A | 2× Super Taq Plus PCR Master Mix with Dye (for Complex/High GC Template Amplification) | 5mL |
BL1309B | 2× Super Taq Plus PCR Master Mix without Dye (for Complex/High GC Template Amplification) | 10×1mL |
HotStart PCR Master Mix
- Hot-start PCR reaction effectively suppresses non-specific amplification and increases target fragment yield - Superior performance: Suitable for high-sensitivity and high-background genomic DNA amplification - Easier operation: Ready-to-use premix, optional red dye for easy identification |
Cat. No. | Description | Packaging |
BL1591A | 2× HotStart Anti-Taq Plus PCR Master Mix with Dye | 1mL |
BL1591B | 2× HotStart Anti-Taq Plus PCR Master Mix with Dye | 10×1mL |
BL1592A | 2× HotStart Anti-Taq Plus PCR Master Mix without Dye | 1mL |
BL1592B | 2× HotStart Anti-Taq Plus PCR Master Mix without Dye | 10×1mL |
BL1593A | 2× HotStart Taq Plus PCR Master Mix with Dye | 1mL |
BL1593B | 2× HotStart Taq Plus PCR Master Mix with Dye | 10×1mL |
BL1594A | 2× HotStart Taq Plus PCR Master Mix without Dye | 1mL |
BL1594B | 2× HotStart Taq Plus PCR Master Mix without Dye | 10×1mL |
HiFi PCR Master Mix
- Easy operation: Only requires the addition of template and primers - High fidelity: 80-fold higher fidelity than wild-type Taq polymerase - Fast extension: Extension speed of approximately 15‒30 s/kb, not exceeding 1 min/kb - Wide application: Suitable for complex template amplification, gene cloning, high-throughput sequencing, site-directed mutagenesis, and more |
Cat. No. | Description | Packaging |
BL1421A | 2 × HiFi Plus PCR Master Mix with Dye | 1mL |
BL1421B | 2 × HiFi Plus PCR Master Mix with Dye | 5×1mL |
BL1421C | 2 × HiFi Plus PCR Master Mix with Dye | 10×1mL |
BL1422A | 2 × HiFi Plus PCR Master Mix | 1mL |
BL1422B | 2 × HiFi Plus PCR Master Mix | 5×1mL |
BL1422C | 2 × HiFi Plus PCR Master Mix | 10×1mL |
BL1603A | HiFi Plus High-Fidelity DNA Polymerase | 100U |
BL1603B | HiFi Plus High-Fidelity DNA Polymerase | 100U×5 |
BL5137A | BL5137A 2× HiFi Max PCR Master Mix with Dye | 1mL |
BL5137B | BL5137B 2× HiFi Max PCR Master Mix with Dye | 5×1mL |
BL5137C | BL5137C 2× HiFi Max PCR Master Mix with Dye | 10×1mL |
BL5138A | BL5138A 2× HiFi Max PCR Master Mix | 1mL |
BL5138B | BL5138B 2× HiFi Max PCR Master Mix | 5×1mL |
BL5138C | BL5138C 2× HiFi Max PCR Master Mix | 10×1mL |
Random Mutagenesis Kit
Based on error-prone PCR technology, which takes advantage of the lack of 3′→5′ proofreading activity of Taq DNA polymerase, random
mutations are introduced into the target gene under specific reaction buffer conditions. The amplified products containing random
mutations are double-digested and ligated into expression vectors to construct a library, which is then transformed into an expression
host for protein activity screening. A sequential error-prone PCR strategy can be used, in which repeated cycles of random mutagenesis
allow mutations to accumulate across rounds, leading to more significant mutations.
Cat. No. | Description | Packaging |
BL1668A | Random Mutagenesis Kit | 20T |
Mouse Tissue Direct PCR Kit
- Direct and rapid PCR amplification from mouse tissue samples - Hot-start high-fidelity DNA polymerase offers high fidelity, high sensitivity, strong specificity, and fast amplification speed - Contains dye, allowing direct electrophoresis after reaction for convenient and fast use - Amplification products are blunt-ended, suitable for blunt-end cloning |
Cat. No. | Description | Packaging |
BL1028A | Mouse Genotyping Rapid Identification Kit | 500 T |
BL1028S | Mouse Genotyping Rapid Identification Kit | 100 T |
PCR (Polymerase Chain Reaction) is a technique for in vitro amplification of specific DNA sequences. The principle involves the replication of complementary DNA strands from a template DNA using DNA polymerase and specific primers through cycles of denaturation, annealing, and extension. It enables rapid and specific amplification of target DNA and is widely used in gene isolation and cloning, sequence analysis, gene expression regulation, and polymorphism studies.
Taq plus PCR Mix
- High efficiency: Amplifies up to 8 kb with λDNA template, efficiently amplifies fragments ≤4 kb - High sensitivity: Amplifies specific gene fragments from 0.05 ng human genomic DNA template - Stable: Maintains amplification performance after repeated freeze-thaw cycles, 30 days at 4°C, or one week at room temperature - Fast: All necessary reagents are provided in a single tube, allowing reaction setup within minutes - Convenient: Direct electrophoresis after PCR reaction |
Experimental Data
A. Amplification detection of mouse tail samples.
Samples 1‒5 represent different brands, with B1 as BL553A and B2 as BL547A. Both enzymes produced clear bands and high amplification yields with different primer sets.
B. Amplification detection of rice samples.
P1‒P7 represent amplification primers for different product sizes. Amplification was stable, with clear bands and high yields across all primers.
Cat. No. | Description | Packaging |
BL553A | 2× Taq Plus PCR Master Mix with Dye | 1mL |
BL553B | 2× Taq Plus PCR Master Mix with Dye | 1mL×5 |
BL553C | 2× Taq Plus PCR Master Mix with Dye | 1mL×25 |
BL553D | 2× Taq Plus PCR Master Mix with Dye | 1mL×100 |
BL1361A | 2× Taq Plus PCR Master Mix without Dye | 1mL |
BL1361B | 2× Taq Plus PCR Master Mix without Dye | 5×1mL |
BL1361C | 2× Taq Plus PCR Master Mix without Dye | 25×1mL |
BL1361D | 2× Taq Plus PCR Master Mix without Dye | 100×1mL |
Fast Taq plus Master Mix
- Faster amplification: 5s/kb for routine templates below 3kb, up to 1s/kb; PCR completed within 30 minutes - Wider applicability: Suitable for templates with high GC content and complex secondary structures - Superior performance: High specificity and yield - Easier operation: Ready-to-use premix, optional red dye for easy identification |
Cat. No. | Description | Packaging |
BL971A | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification) | 1mL |
BL971B | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification) | 10×1mL |
BL971C | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification) | 100×1mL |
BL1014A | 2× Fast Taq Plus PCR Master Mix without Dye (for Rapid Amplification) | 1mL |
BL1014B | 2× Fast Taq Plus PCR Master Mix without Dye (for Rapid Amplification) | 10×1mL |
BL1014C | 2× Fast Taq Plus PCR Master Mix without Dye (for Rapid Amplification) | 100×1mL |
BL1513A | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification, Low Background) | 1mL |
BL1513B | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification, Low Background) | 10×1mL |
BL1513C | 2× Fast Taq Plus PCR Master Mix with Dye (for Rapid Amplification, Low Background) | 100×1mL |
Pfu PCR Mix
- Only requires the addition of primers and template for PCR, minimizing human error, with fast and simple operation and good stability - Used for high-fidelity amplification of DNA fragments - Amplification products are blunt-ended, suitable for blunt-end cloning |
Cat. No. | Description | Packaging |
BL111A | 2× Pfu PCR Master Mix with Dye | 1mL |
Quality Assurance
- Amplifies a 5 kb DNA fragment efficiently using λ DNA as a template
- Amplifies a 3.2 kb DNA fragment efficiently using rice genomic DNA as a template
Taq plus PCR Mix for PAGE
- Direct loading: PCR products with dye can be directly loaded for gel electrophoresis - High sensitivity: High-purity enzyme ensures excellent sensitivity - No genomic contamination: Amplified products are of high purity - Efficient amplification: Optimized buffer system enhances amplification performance - Good reproducibility: Premixed system reduces pipetting errors and contamination risk - Batch-to-batch consistency: Standardized production process and strict quality control |
Cat. No. | Description | Packaging |
BL631A | 2× Taq PCR Master Mix for PAGE with Dye | 1mL |
BL631B | 2× Taq PCR Master Mix for PAGE with Dye | 5×1mL |
BL634A | 50 bp DNA Marker (50‒600 bp) | 1mL |
BL1514A | 2× Super Taq PCR Master Mix for PAGE with Dye (for Rapid Amplification) | 1mL |
BL1514B | 2× Super Taq PCR Master Mix for PAGE with Dye (for Rapid Amplification) | 5×1mL |
Super Taq PCR Premix
- Possesses 5′→3′ DNA polymerase activity and 3′→5′ exonuclease activity - 6-fold higher fidelity than standard Taq DNA Polymerase, with high sensitivity and good stability - Strong synthesis capability, suitable for amplification of complex templates and rapid amplification - Pre-mixed PCR format minimizes human error, saves time, and reduces contamination risk - PCR products have a 3′-terminal overhang "A" base, allowing direct cloning into T-vectors |
Cat. No. | Description | Packaging |
BL1015A | 2× Super Taq Plus PCR Master Mix with Dye (for Complex/High GC Template Amplification) | 1mL |
BL1015B | 2× Super Taq Plus PCR Master Mix with Dye (for Complex/High GC Template Amplification) | 5×1mL |
BL1015C | 2× Super Taq Plus PCR Master Mix with Dye (for Complex/High GC Template Amplification) | 10×1mL |
BL1309A | 2× Super Taq Plus PCR Master Mix without Dye (for Complex/High GC Template Amplification) | 1mL |
BL5824A | 2× Super Taq Plus PCR Master Mix with Dye (for Complex/High GC Template Amplification) | 5mL |
BL1309B | 2× Super Taq Plus PCR Master Mix without Dye (for Complex/High GC Template Amplification) | 10×1mL |
HotStart PCR Master Mix
- Hot-start PCR reaction effectively suppresses non-specific amplification and increases target fragment yield - Superior performance: Suitable for high-sensitivity and high-background genomic DNA amplification - Easier operation: Ready-to-use premix, optional red dye for easy identification |
Cat. No. | Description | Packaging |
BL1591A | 2× HotStart Anti-Taq Plus PCR Master Mix with Dye | 1mL |
BL1591B | 2× HotStart Anti-Taq Plus PCR Master Mix with Dye | 10×1mL |
BL1592A | 2× HotStart Anti-Taq Plus PCR Master Mix without Dye | 1mL |
BL1592B | 2× HotStart Anti-Taq Plus PCR Master Mix without Dye | 10×1mL |
BL1593A | 2× HotStart Taq Plus PCR Master Mix with Dye | 1mL |
BL1593B | 2× HotStart Taq Plus PCR Master Mix with Dye | 10×1mL |
BL1594A | 2× HotStart Taq Plus PCR Master Mix without Dye | 1mL |
BL1594B | 2× HotStart Taq Plus PCR Master Mix without Dye | 10×1mL |
HiFi PCR Master Mix
- Easy operation: Only requires the addition of template and primers - High fidelity: 80-fold higher fidelity than wild-type Taq polymerase - Fast extension: Extension speed of approximately 15‒30 s/kb, not exceeding 1 min/kb - Wide application: Suitable for complex template amplification, gene cloning, high-throughput sequencing, site-directed mutagenesis, and more |
Cat. No. | Description | Packaging |
BL1421A | 2 × HiFi Plus PCR Master Mix with Dye | 1mL |
BL1421B | 2 × HiFi Plus PCR Master Mix with Dye | 5×1mL |
BL1421C | 2 × HiFi Plus PCR Master Mix with Dye | 10×1mL |
BL1422A | 2 × HiFi Plus PCR Master Mix | 1mL |
BL1422B | 2 × HiFi Plus PCR Master Mix | 5×1mL |
BL1422C | 2 × HiFi Plus PCR Master Mix | 10×1mL |
BL1603A | HiFi Plus High-Fidelity DNA Polymerase | 100U |
BL1603B | HiFi Plus High-Fidelity DNA Polymerase | 100U×5 |
BL5137A | BL5137A 2× HiFi Max PCR Master Mix with Dye | 1mL |
BL5137B | BL5137B 2× HiFi Max PCR Master Mix with Dye | 5×1mL |
BL5137C | BL5137C 2× HiFi Max PCR Master Mix with Dye | 10×1mL |
BL5138A | BL5138A 2× HiFi Max PCR Master Mix | 1mL |
BL5138B | BL5138B 2× HiFi Max PCR Master Mix | 5×1mL |
BL5138C | BL5138C 2× HiFi Max PCR Master Mix | 10×1mL |
Random Mutagenesis Kit
Based on error-prone PCR technology, which takes advantage of the lack of 3′→5′ proofreading activity of Taq DNA polymerase, random
mutations are introduced into the target gene under specific reaction buffer conditions. The amplified products containing random
mutations are double-digested and ligated into expression vectors to construct a library, which is then transformed into an expression
host for protein activity screening. A sequential error-prone PCR strategy can be used, in which repeated cycles of random mutagenesis
allow mutations to accumulate across rounds, leading to more significant mutations.
Cat. No. | Description | Packaging |
BL1668A | Random Mutagenesis Kit | 20T |
Mouse Tissue Direct PCR Kit
- Direct and rapid PCR amplification from mouse tissue samples - Hot-start high-fidelity DNA polymerase offers high fidelity, high sensitivity, strong specificity, and fast amplification speed - Contains dye, allowing direct electrophoresis after reaction for convenient and fast use - Amplification products are blunt-ended, suitable for blunt-end cloning |
Cat. No. | Description | Packaging |
BL1028A | Mouse Genotyping Rapid Identification Kit | 500 T |
BL1028S | Mouse Genotyping Rapid Identification Kit | 100 T |
