Mitochondria are the powerhouses of the cell, where oxidative phosphorylation takes place. The energy required for cellular activities is primarily supplied by oxidation reactions occurring within mitochondria. The key to preparing mitochondria is maintaining their integrity and purity, which can be achieved through differential centrifugation: first removing nuclei and cell debris by low-speed centrifugation, followed by high-speed gradient centrifugation to isolate mitochondria. Isolating mitochondria is significant for studying mitochondrial respiration, mtDNA, mtRNA, and the functional analysis of other mitochondrial proteins. Our company currently offers cell and tissue mitochondria isolation kits for rapid and convenient isolation of mitochondria from animal cells or tissues. In addition to isolating mitochondria, these kits also allow collection of mitochondria-depleted cytosolic proteins, which can be used to analyze the release of mitochondrial proteins such as cytochrome c into the cytoplasm. Most isolated mitochondria retain intact inner and outer membranes and maintain physiological functions (such as detecting mitochondrial membrane potential). The obtained proteins are suitable for protein analysis including SDS-PAGE, Western blot, and two-dimensional electrophoresis.
Cat. No. | Description | Packaging |
BL153A | Tissue Mitochondria Isolation Kit | 50T |
BL168A | Cell Mitochondria Isolation Kit | 50T |
Mitochondria are the powerhouses of the cell, where oxidative phosphorylation takes place. The energy required for cellular activities is primarily supplied by oxidation reactions occurring within mitochondria. The key to preparing mitochondria is maintaining their integrity and purity, which can be achieved through differential centrifugation: first removing nuclei and cell debris by low-speed centrifugation, followed by high-speed gradient centrifugation to isolate mitochondria. Isolating mitochondria is significant for studying mitochondrial respiration, mtDNA, mtRNA, and the functional analysis of other mitochondrial proteins. Our company currently offers cell and tissue mitochondria isolation kits for rapid and convenient isolation of mitochondria from animal cells or tissues. In addition to isolating mitochondria, these kits also allow collection of mitochondria-depleted cytosolic proteins, which can be used to analyze the release of mitochondrial proteins such as cytochrome c into the cytoplasm. Most isolated mitochondria retain intact inner and outer membranes and maintain physiological functions (such as detecting mitochondrial membrane potential). The obtained proteins are suitable for protein analysis including SDS-PAGE, Western blot, and two-dimensional electrophoresis.
Cat. No. | Description | Packaging |
BL153A | Tissue Mitochondria Isolation Kit | 50T |
BL168A | Cell Mitochondria Isolation Kit | 50T |
