Lipophilic carbocyanine dyes are commonly used for cell membrane staining, cell tracing, and subcellular localization. DiO, DiI, and DiD are commonly used fluorescent probes. After entering the cell membrane, they undergo lateral diffusion to gradually stain the entire cell membrane, and the staining remains very stable. These dyes exhibit very weak fluorescence before entering the cell membrane, but become strongly fluorescent only upon membrane incorporation. DiO emits green fluorescence; when bound to phospholipid bilayers, its maximum excitation wavelength is 484 nm and maximum emission wavelength is 501 nm. DiI emits orange-red fluorescence; when bound to phospholipid bilayers, its maximum excitation wavelength is 549 nm and maximum emission wavelength is 565 nm. DiD is an analog of DiI with longer excitation and emission wavelengths, emitting far-red fluorescence; when bound to phospholipid bilayers, its maximum excitation wavelength is 644 nm and maximum emission wavelength is 665 nm.
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The figure shows DiI staining of EPCs. | The figure shows DiO staining of HeLa cells. | The figure shows DiD staining of rat lymphocytes. |
Cat. No. | Description | Packaging |
BL776A | Cell Membrane Red Fluorescence Staining Kit (DiI) | 200T-1000T |
BL777A | Cell Membrane Green Fluorescence Staining Kit (DiO) | 200T-1000T |
BL927A | Cell Membrane Far-Red Fluorescence Staining Kit (DiD) | 200T-1000T |
BL926A | DiD (Cell Membrane Far-Red Fluorescent Probe) | 10mg |
BL925A | DiO (Cell Membrane Green Fluorescent Probe) | 10mg |
BL1031A | DiI (Cell Membrane Red Fluorescent Probe) | 10mg |
Lipophilic carbocyanine dyes are commonly used for cell membrane staining, cell tracing, and subcellular localization. DiO, DiI, and DiD are commonly used fluorescent probes. After entering the cell membrane, they undergo lateral diffusion to gradually stain the entire cell membrane, and the staining remains very stable. These dyes exhibit very weak fluorescence before entering the cell membrane, but become strongly fluorescent only upon membrane incorporation. DiO emits green fluorescence; when bound to phospholipid bilayers, its maximum excitation wavelength is 484 nm and maximum emission wavelength is 501 nm. DiI emits orange-red fluorescence; when bound to phospholipid bilayers, its maximum excitation wavelength is 549 nm and maximum emission wavelength is 565 nm. DiD is an analog of DiI with longer excitation and emission wavelengths, emitting far-red fluorescence; when bound to phospholipid bilayers, its maximum excitation wavelength is 644 nm and maximum emission wavelength is 665 nm.
| | |
The figure shows DiI staining of EPCs. | The figure shows DiO staining of HeLa cells. | The figure shows DiD staining of rat lymphocytes. |
Cat. No. | Description | Packaging |
BL776A | Cell Membrane Red Fluorescence Staining Kit (DiI) | 200T-1000T |
BL777A | Cell Membrane Green Fluorescence Staining Kit (DiO) | 200T-1000T |
BL927A | Cell Membrane Far-Red Fluorescence Staining Kit (DiD) | 200T-1000T |
BL926A | DiD (Cell Membrane Far-Red Fluorescent Probe) | 10mg |
BL925A | DiO (Cell Membrane Green Fluorescent Probe) | 10mg |
BL1031A | DiI (Cell Membrane Red Fluorescent Probe) | 10mg |
