Compared with conventional chemical dyes, fluorescent dyes offer high sensitivity, strong specificity, and simple operation. They are widely used in fluorescence immunoassays, fluorescent probes, and cell staining, including specific DNA staining for chromosome analysis, cell cycle, apoptosis, and related studies. They can be used individually to stain the nucleus, cell membrane, intracellular components, and organelles, or combined into multiplex fluorescent dyes for simultaneous staining of different targets, yielding clearer and more accurate results. DAPI, PI, and Hoechst 33258/33342 are common DNA dyes primarily used for nuclear staining, cell cycle analysis by fluorescence intensity, and background labeling. Fluorescent probes are mainly used to detect specific intracellular substances and organelles, aiding studies on cellular physiological functions and structural effects.
DAPI Staining Solution
DAPI Staining Solution is suitable for nuclear staining in common cells and tissues. DAPI is a cell-permeable blue fluorescent dye for live and fixed cells. DAPI has a maximum excitation wavelength of 340 nm and maximum emission wavelength of 488 nm; when bound to double-stranded DNA, the maximum excitation shifts to 364 nm and maximum emission to 454 nm. DAPI emits blue fluorescence, and its emission spectrum has minimal overlap with GFP or Texas Red, allowing multiplex fluorescence staining in a single sample.
PI Staining Solution
Propidium iodide (PI) cannot penetrate intact cell membranes but can enter late apoptotic and dead cells with compromised membranes, staining the nucleus red. It is often used in combination with fluorescent probes such as Calcein-AM or FDA to distinguish live and dead cells based on staining results. The PI-DNA complex has excitation and emission wavelengths of 535 nm and 615 nm, respectively, and is commonly analyzed by flow cytometry or fluorescence microscopy.
Hoechst Staining Solution
Hoechst dyes are fluorescent dyes used to label DNA in microscopic observation. Because they bind to DNA, they are commonly used for visualizing nuclei and mitochondria. Two related dyes, Hoechst 33258 and Hoechst 33342, are frequently used. Both dyes are excited at 350 nm under UV light and emit blue/cyan fluorescence near 461 nm. Hoechst dyes can be used for live or fixed cells and are often used as an alternative to other nucleic acid dyes such as DAPI.
Cat. No. | Description | Packaging |
BL105A | DAPI Solution (Ready-to-Use) | 10mL |
BL105B | DAPI Solution (Ready-to-Use) | 50mL |
BL120A | DAPI Solution (5mg/mL) | 0.2mL |
BL708A | Propidium Iodide (PI) Solution (1mg/mL) | 1mL |
BL804A | Hoechst 33258 Staining Solution (Ready-to-Use, 10μg/mL) | 10mL |
BL803A | Hoechst 33342 Staining Solution (Ready-to-Use, 10μg/mL) | 10mL |
BL1145A | Hoechst 33342 Cell Staining Solution (100×, 1mg/mL) | 0.5mL |
BL1145B | Hoechst 33342 Cell Staining Solution (100×, 1mg/mL) | 1mL |
BL1145C | Hoechst 33342 Cell Staining Solution (100×, 1mg/mL) | 1mL×5 |
Compared with conventional chemical dyes, fluorescent dyes offer high sensitivity, strong specificity, and simple operation. They are widely used in fluorescence immunoassays, fluorescent probes, and cell staining, including specific DNA staining for chromosome analysis, cell cycle, apoptosis, and related studies. They can be used individually to stain the nucleus, cell membrane, intracellular components, and organelles, or combined into multiplex fluorescent dyes for simultaneous staining of different targets, yielding clearer and more accurate results. DAPI, PI, and Hoechst 33258/33342 are common DNA dyes primarily used for nuclear staining, cell cycle analysis by fluorescence intensity, and background labeling. Fluorescent probes are mainly used to detect specific intracellular substances and organelles, aiding studies on cellular physiological functions and structural effects.
DAPI Staining Solution
DAPI Staining Solution is suitable for nuclear staining in common cells and tissues. DAPI is a cell-permeable blue fluorescent dye for live and fixed cells. DAPI has a maximum excitation wavelength of 340 nm and maximum emission wavelength of 488 nm; when bound to double-stranded DNA, the maximum excitation shifts to 364 nm and maximum emission to 454 nm. DAPI emits blue fluorescence, and its emission spectrum has minimal overlap with GFP or Texas Red, allowing multiplex fluorescence staining in a single sample.
PI Staining Solution
Propidium iodide (PI) cannot penetrate intact cell membranes but can enter late apoptotic and dead cells with compromised membranes, staining the nucleus red. It is often used in combination with fluorescent probes such as Calcein-AM or FDA to distinguish live and dead cells based on staining results. The PI-DNA complex has excitation and emission wavelengths of 535 nm and 615 nm, respectively, and is commonly analyzed by flow cytometry or fluorescence microscopy.
Hoechst Staining Solution
Hoechst dyes are fluorescent dyes used to label DNA in microscopic observation. Because they bind to DNA, they are commonly used for visualizing nuclei and mitochondria. Two related dyes, Hoechst 33258 and Hoechst 33342, are frequently used. Both dyes are excited at 350 nm under UV light and emit blue/cyan fluorescence near 461 nm. Hoechst dyes can be used for live or fixed cells and are often used as an alternative to other nucleic acid dyes such as DAPI.
Cat. No. | Description | Packaging |
BL105A | DAPI Solution (Ready-to-Use) | 10mL |
BL105B | DAPI Solution (Ready-to-Use) | 50mL |
BL120A | DAPI Solution (5mg/mL) | 0.2mL |
BL708A | Propidium Iodide (PI) Solution (1mg/mL) | 1mL |
BL804A | Hoechst 33258 Staining Solution (Ready-to-Use, 10μg/mL) | 10mL |
BL803A | Hoechst 33342 Staining Solution (Ready-to-Use, 10μg/mL) | 10mL |
BL1145A | Hoechst 33342 Cell Staining Solution (100×, 1mg/mL) | 0.5mL |
BL1145B | Hoechst 33342 Cell Staining Solution (100×, 1mg/mL) | 1mL |
BL1145C | Hoechst 33342 Cell Staining Solution (100×, 1mg/mL) | 1mL×5 |
