The Reactive Oxygen Species (ROS) Assay Kit uses the fluorescent probe H2DCFDA to detect ROS levels. H2DCFDA is non-fluorescent and cell-permeable. Once inside cells, it is hydrolyzed by intracellular esterases to form DCFH, which is membrane-impermeable and retained within cells. Intracellular ROS oxidize non-fluorescent DCFH to produce fluorescent DCF. Fluorescence intensity correlates with ROS levels, allowing assessment of ROS content and changes in live cells. Detection can be performed by flow cytometry or fluorescence microscopy, making it a classic method for ROS detection.
Cat. No. | Description | Packaging |
BL714A | ROS Assay Kit | 1000T |
BL714B | ROS Assay Kit | 5000T |
Total SOD Activity Assay Kits are available in three formats: NBT, WST-1, and WST-8. NBT is the traditional method, while WST-1 and WST-8 are improved versions with higher sensitivity and stability. All three methods share a similar principle: superoxide anions generated by xanthine oxidase (XO) react with the reagents to form water-soluble formazan dye. SOD inhibits this reaction by catalyzing the dismutation of superoxide anions, so SOD activity is inversely correlated with formazan production. SOD activity can thus be calculated by colorimetric analysis. These kits incorporate catalase and other components to effectively eliminate interference from hydrogen peroxide in routine samples. For instance, the presence of up to 0.1 mM hydrogen peroxide in SOD standard samples does not significantly affect detection results. All three kits are suitable for measuring SOD activity in various biological samples, including cell or tissue homogenate supernatants, whole blood, erythrocyte extracts, and serum, offering broad applicability.
Cat. No. | Description | Packaging |
BL901A | Total SOD Activity Assay Kit (NBT Method) | 100T |
BL902A | Total SOD Activity Assay Kit (WST-1 Method) | 100T |
BL903A | Total SOD Activity Assay Kit (WST-8 Method) | 100T |
Lipid Peroxidation (MDA) Assay Kit is based on the colorimetric reaction between malondialdehyde (MDA) and thiobarbituric acid (TBA), which produces a red product. It is used for quantitative detection of MDA in plasma, serum, urine, animal/plant tissues, or cell lysates, making it widely used for assessing lipid peroxidation levels. This kit can detect MDA concentrations ranging from as low as 1 μM to as high as 200 μM. MDA levels in plasma and serum are typically around 2‒4 μM, while urine levels range from approximately 5‒30 μM, all within the detection range of this kit. Plasma, serum, and urine samples can be directly tested.
Cat. No. | Description | Packaging |
BL904A/B | Lipid Peroxidation (MDA) Assay Kit | 100T/500T |
The Senescence β-Galactosidase Staining Kit detects cellular senescence by measuring the activity of senescence-associated β-galactosidase (SA-β-Gal). In senescent cells, SA-β-Gal is active at pH 6.0 and catalyzes the substrate X-Gal to produce a blue product, resulting in blue deposits in the cytoplasm. Stained cells or tissues can be easily observed under light microscopy. This kit is suitable for senescence detection in cultured cells and tissue sections. It specifically stains senescent cells and does not stain presenescent cells, quiescent cells, immortal cells, or tumor cells.
Cat. No. | Description | Packaging |
BL133A | Senescence β-Galactosidase Staining Kit | 100T |
The Autophagy Staining Detection Kit (MDC Method) uses monodansylcadaverine (MDC) as a fluorescent probe for rapid and convenient detection of autophagy. Autophagy is a critical physiological process in eukaryotes for intracellular material turnover. It involves the formation of autophagosomes, which can be specifically labeled by MDC, an acidophilic fluorescent dye. This kit is suitable for fluorescence-based autophagy staining in cultured cells and can also be used for FACS (Fluorescence Activated Cell Sorting) analysis to quantify autophagy. Also known as MDC Staining Solution, it can be combined with EB for double staining.
Cat. No. | Description | Packaging |
BL1139A | Autophagy Staining Detection Kit (MDC Method) | 100T |
The Caspase-3 Activity Assay Kit uses spectrophotometry to measure caspase-3 enzyme activity in cell or tissue lysates or purified caspase-3 samples. Caspases are a family of proteases that play a key role in apoptosis. Caspase-3, also known as CPP32, Yama, or apopain, belongs to the CED-3 subfamily and is a crucial enzyme in apoptosis, being the most extensively studied caspase in mammalian cells. This kit is based on the cleavage of the substrate Ac-DEVD-pNA by caspase-3, releasing yellow pNA (p-nitroaniline). Caspase-3 activity is determined by measuring absorbance.
Cat. No. | Description | Packaging |
BL1212A/B | Caspase-3 Activity Assay Kit | 20T/100T |
LDH Cytotoxicity Assay Kit is based on a diaphorase-catalyzed INT colorimetric reaction, used to measure LDH activity released during cytotoxicity or in other samples. This kit is suitable for routine LDH activity detection and is commonly used for cytotoxicity assays based on LDH release. It can also be used for cell proliferation and cytotoxicity detection by measuring total cellular LDH activity.
Cat. No. | Description | Packaging |
BL1405A/B | LDH Cytotoxicity Assay Kit | 100T/500T |
Mycoplasma Stain Assay Kit is a classic DNA fluorescent staining method for in situ detection of mycoplasma or other prokaryotes in cultured cells, primarily used to identify mycoplasma contamination. The principle relies on the fluorescent dye bisbenzimide (Hoechst 33258), which binds to A-T rich regions of DNA. Since mycoplasma DNA has a high A-T content (55%‒80%), it can be specifically stained and detected. In contaminated cultures, numerous uniformly sized blue fluorescent dots are visible around the cells, indicating mycoplasma contamination.
Cat. No. | Description | Packaging |
BL1116A | Mycoplasma Stain Assay Kit | 100T |
Mycoplasma Detection Kit (PCR Method) uses PCR to specifically detect mycoplasma contamination in cultured cells and biological materials such as cell culture supernatants, experimental animal secretions, and animal serum. The kit employs mixed primers designed against the conserved region of the mycoplasma 16S rRNA sequence, allowing direct use of cell culture medium as the PCR template for specific amplification of mycoplasma DNA. Detection can be completed within one hour, with high sensitivity capable of detecting as few as 20 copies of mycoplasma DNA.
Cat. No. | Description | Packaging |
BL1469A | Mycoplasma Detection Kit (PCR Method) | 50T |
Luciferase Reporter Gene Assay Kit is based on firefly luciferase (~61 kDa), which catalyzes the oxidation of luciferin to oxyluciferin in the presence of ATP, Mg²⁺, and oxygen, producing bioluminescence at ~560 nm. Renilla luciferase (~36 kDa) catalyzes the oxidation of coelenterazine to coelenteramide in the presence of oxygen, producing bioluminescence at ~480 nm. Bioluminescence can be measured using a luminometer or liquid scintillation counter. This system provides a highly sensitive and efficient method for gene expression analysis. Typically, the transcriptional regulatory element or 5′ promoter region of a gene of interest is cloned upstream of the luciferase reporter gene, or the 3′-UTR is cloned downstream, to construct a reporter plasmid. After transfection and appropriate treatment, cells are lysed, and luciferase activity is measured to assess transcriptional regulation. Renilla luciferase is commonly used as an internal control to normalize for variations in transfection efficiency and cell number.
Cat. No. | Description | Packaging |
BL555A/B | Dual-Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1604A/B | Bright-Luc Firefly Luciferase Reporter Gene Assay Kit (Pre-Mixed) | 100T/1000T |
BL1605A/B | Bright-Luc Firefly Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1606A/B | One-Luc Firefly Luciferase Reporter Gene Assay Kit (Pre-Mixed) | 100T/1000T |
BL1607A/B | One-Luc Firefly Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1608A/B | Renilla-Luc Renilla Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1609A/B | Dual-Luc Dual-Luciferase Reporter Gene Assay Kit (Pre-Mixed) | 100T/1000T |
BL1610A/B | Dual-Luc Dual-Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1611A/B | Firefly Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1613A/B | Firefly Luciferase Reporter Gene Cell Lysis Buffer | 10mL/100mL |
BL1614A/B | Firefly Luciferase Reporter Gene Cell Lysis Buffer (Enhanced) | 10mL/100mL |
BL1612A/B | Renilla Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1615A/B | Renilla Luciferase Reporter Gene Cell Lysis Buffer | 10mL/100mL |
BL1616A/B | Dual-Luciferase Reporter Gene Cell Lysis Buffer | 10mL/100mL |
BL5561A | Firefly Luciferase Reporter Gene Assay Kit (Enhanced) | 100T |
BL5561B | Firefly Luciferase Reporter Gene Assay Kit (Enhanced) | 1000T |
BL5562A | Firefly Luciferase Reporter Gene Assay Kit (Stable) | 100T |
BL5562B | Firefly Luciferase Reporter Gene Assay Kit (Stable) | 1000T |
BL5563A | Renilla Luciferase Reporter Gene Assay Kit | 100T |
BL5563B | Renilla Luciferase Reporter Gene Assay Kit | 1000T |
BL5564A | Dual-Luciferase Reporter Gene Assay Kit | 100T |
BL5564B | Dual-Luciferase Reporter Gene Assay Kit | 1000T |
β-Galactosidase Reporter Gene Assay Kit is used for β-galactosidase reporter gene detection. β-Galactosidase is a commonly used reporter gene, often co-transfected with luciferase reporters as an internal control to normalize for variations in transfection efficiency. This kit uses colorless ONPG as a substrate, which is catalyzed by β-galactosidase to produce yellow o-nitrophenol. Absorbance is measured at 420 nm using a microplate reader or spectrophotometer to determine β-galactosidase activity.
Cat. No. | Description | Packaging |
BL1617A | β-Galactosidase Reporter Gene Assay Kit | 200T |
Mycoplasma Detection Kit (Chemiluminescent Method) is based on the activity of an enzyme unique to mycoplasma. This enzyme cleaves a specific substrate in the detection reagent while converting ADP to ATP. Luciferase then catalyzes luciferin oxidation in the presence of ATP, producing bioluminescence that can be measured by a luminometer to determine whether a sample is contaminated with mycoplasma. The assay requires only two steps and takes approximately 15 minutes. It offers high sensitivity by detecting metabolically active mycoplasma, making results more accurate than PCR-based methods.
Cat. No. | Description | Packaging |
BL1470A | Mycoplasma Detection Kit (Chemiluminescent Method) | 20T |
Mycoplasma Detection Kit (Isothermal Amplification) uses isothermal amplification with visual color change to rapidly detect 8 common mycoplasma species in cell culture media. No DNA extraction is required; simply add 1 μL of cell culture supernatant to the reaction mixture and incubate at 65°C for 30 min. Positive samples change from yellow to red. The process requires no PCR instrument or post-amplification electrophoresis, effectively preventing cross-contamination from aerosols. Compared to traditional PCR, this kit is simple to operate, highly sensitive, and tolerates various PCR inhibitors present in culture media, eliminating false negatives. Results show high consistency with qPCR.
Cat. No. | Description | Packaging |
BL1471A | One-Step Rapid Mycoplasma Detection Kit (Isothermal Amplification) | 50T |
NAD⁺/NADH Assay Kit uses WST-8 colorimetric reaction to measure NAD⁺ (oxidized nicotinamide adenine dinucleotide) and NADH (reduced nicotinamide adenine dinucleotide) levels, ratio, and total amount in cells, tissues, or other samples. The kit specifically detects NAD⁺ and NADH without interference from NADP⁺ and NADPH. During the reaction, NAD⁺ is reduced to NADH, which then reduces WST-8 to orange-yellow formazan with maximum absorbance at ~450 nm. The amount of formazan produced is proportional to the total amount of NAD⁺ or NADH in the sample.
Cat. No. | Description | Packaging |
BL1431A | NAD+/NADH Assay Kit | 100T |
NADP⁺/NADPH Assay Kit uses WST-8 colorimetric reaction to measure NADP⁺ (nicotinamide adenine dinucleotide phosphate, oxidized) and NADPH (reduced) levels, ratio, and total amount in cells, tissues, or other samples. The kit specifically detects NADP⁺ and NADPH without interference from NAD⁺ and NADH. During reaction, NADP⁺ is reduced to NADPH, which then reduces WST-8 to orange-yellow formazan with maximum absorbance at ~450 nm. Formazan production is proportional to total NADP⁺ or NADPH in sample.
Cat. No. | Description | Packaging |
BL1432A | NADP+/NADPH Assay Kit | 100T |
ATP Assay Kit is based on firefly luciferase catalyzing luciferin to produce fluorescence, which requires ATP for energy. When both firefly luciferase and luciferin are in excess, fluorescence production is proportional to ATP concentration within a certain range, enabling highly sensitive detection of ATP levels in solution.
Cat. No. | Description | Packaging |
BL1554A | ATP Assay Kit (1nM) | 200T |
BL1555A | Enhanced ATP Assay Kit (0.1nM) | 200T |
CellTiter-Flu Luminescent Cell Viability Assay Kit is based on luciferase catalyzing luciferin, ATP, and O₂ in the presence of Mg²⁺, converting chemical energy into light. In this reaction, ATP concentration is linearly correlated with luminescence intensity within a certain range. Luminescence signal is proportional to ATP amount, which is directly proportional to cell number in culture.
Cat. No. | Description | Packaging |
BL1618A | CellTiter-Flu Luminescent Cell Viability Assay Kit | 100T |
BL1618B | CellTiter-Flu Luminescent Cell Viability Assay Kit | 1000T |
BL1618C | CellTiter-Flu Luminescent Cell Viability Assay Kit | 5000T |
Hydrogen Peroxide Assay Kit is based on the oxidation of ferrous ions to ferric ions by hydrogen peroxide, which then form a purple complex with xylenol orange in a specific solution, enabling determination of hydrogen peroxide concentration. This improved formulation can detect as low as 1 μmol/L hydrogen peroxide.
Cat. No. | Description | Packaging |
BL1556A | Hydrogen Peroxide Assay Kit | 150T |
Glutathione Assay Kit uses glutathione reductase to reduce oxidized glutathione (GSSG) to reduced glutathione (GSH). GSH then reacts with the chromogenic substrate DTNB, producing yellow TNB and regenerating GSSG. Under appropriate reaction conditions, total glutathione (GSSG+GSH) becomes the rate-limiting factor for color development, with TNB formation proportional to total glutathione amount. Total glutathione is determined by measuring absorbance at 412 nm.To measure GSSG specifically, GSH is first removed from the sample using a selective reagent, then the same reaction is performed. GSH content is calculated by subtracting GSSG from total glutathione.
Cat. No. | Description | Packaging |
BL1557A | GSH and GSSG Assay Kit | 100T |
BL1558A | Total Glutathione Assay Kit | 100T |
Calcium Colorimetric Assay Kit is a colorimetric method for rapid quantitative detection of calcium ion levels in cells, tissues, serum, plasma, urine, culture media, and other samples. The principle is based on the reaction of calcium ions with o-cresolphthalein complexone under alkaline conditions, forming a purple complex. Calcium concentration is determined by measuring absorbance at 575 nm.
Cat. No. | Description | Packaging |
BL1561A | Calcium Colorimetric Assay Kit | 200T |
Alkaline Phosphatase Assay Kit provides a highly sensitive, simple, and direct method for detecting endogenous alkaline
phosphatase (ALP) activity in cell or tissue lysates, homogenates, plasma, serum, urine, and other samples. The principle is based on
p-nitrophenyl phosphate (pNPP) as a substrate. Under alkaline conditions, ALP cleaves pNPP to produce p-nitrophenol, which forms a
deep yellow quinoid structure with maximum absorbance at 405 nm. Higher color intensity indicates higher ALP activity.
Cat. No. | Description | Packaging |
BL1428A | Alkaline Phosphatase Assay Kit | 100T |
Total Antioxidant Capacity (T-AOC) Assay Kit (ABTS Method) uses ABTS as a chromogenic agent to measure total
antioxidant capacity in plasma, serum, saliva, urine, cell or tissue lysates, plant or herbal extracts, and various antioxidant solutions.
The principle is based on ABTS being oxidized to green ABTS⁺ by appropriate oxidants. Antioxidants inhibit ABTS⁺ formation, and total
antioxidant capacity is determined by measuring absorbance at 734 nm or 405 nm.
Cat. No. | Description | Packaging |
BL1429A | Total Antioxidant Capacity Assay Kit (ABTS Method) | 300T |
Acridine Orange (AO)/PI Dual Fluorescence Staining Kit uses AO/PI dual fluorescence counting to determine cell
concentration and viability. The staining solution contains acridine orange (green fluorescent dye) and propidium iodide (red
fluorescent dye). PI is membrane-impermeable and only enters cells with damaged membranes, while AO penetrates all cells. When
both dyes are present in the nucleus, PI causes reduction in AO fluorescence through fluorescence resonance energy transfer (FRET).
Therefore, intact nucleated cells stain green and are counted as viable, while damaged nucleated cells stain red and are counted as
non-viable.
Cat. No. | Description | Packaging |
BL5565A | Acridine Orange (AO)/PI Dual Fluorescence Staining Kit | 2×5mL |
BL5565B | Acridine Orange (AO)/PI Dual Fluorescence Staining Kit | 2×25mL |
Bacterial Endotoxin Detection Kit (Kinetic Chromogenic Method) is based on bacterial endotoxin specifically
activating factor C in the kit's main reagent. Activated factor C activates factor B, which then activates proclotting enzyme. The
activated clotting enzyme hydrolyzes a chromogenic substrate, releasing free pNA (p-nitroaniline) and causing changes in absorbance.
Endotoxin concentration is quantified by dynamically measuring the rate of absorbance change. It is used for quantitative determination
of bacterial endotoxin in human and animal injectable drugs, biologics, medical devices, and other samples.
Cat. No. | Description | Packaging |
BL5615A | Bacterial Endotoxin Detection Kit (Kinetic Chromogenic Method) 0.01‒10 EU/mL | 96T |
BL5616A | Bacterial Endotoxin Detection Kit (Kinetic Chromogenic Method) 0.005‒10 EU/mL | 96T |
Bacterial Endotoxin Detection Reagent (Gel Clot Method) is a freeze-dried product derived from the amebocyte
lysate of horseshoe crabs, containing proclotting enzyme and coagulogen that can be activated by trace amounts of bacterial
endotoxin. Under suitable conditions (temperature, pH, and absence of interfering substances), bacterial endotoxin activates the
proclotting enzyme in the reagent, causing a clotting reaction that forms a gel. The firmness of the gel determines the limit detection
of bacterial endotoxin. This product appears as a white or off-white lyophilized powder that is readily soluble in water. It is used for
limit testing of bacterial endotoxin in culture media, syringes, dialysis equipment, vaccines, antibiotics, protein drugs, and other
reagents, consumables, and pharmaceuticals.
Cat. No. | Description | Packaging |
BL5610A | Bacterial Endotoxin Detection Reagent (Gel Clot Method) 0.03 EU/mL | 10tests × 10vials/box |
BL5611A | Bacterial Endotoxin Detection Reagent (Gel Clot Method) 0.06 EU/mL | 10tests × 10vials/box |
BL5612A | Bacterial Endotoxin Detection Reagent (Gel Clot Method) 0.125 EU/mL | 10tests × 10vials/box |
BL5613A | Bacterial Endotoxin Detection Reagent (Gel Clot Method) 0.25 EU/mL | 10tests × 10vials/box |
BL5614A | Bacterial Endotoxin Detection Reagent (Gel Clot Method) 0.5 EU/mL | 10tests × 10vials/box |
BL5617A | Bacterial Endotoxin Working Standard (CSE) 10‒100 EU/vial | 10vials/box |
BL5618A | Endotoxin Indicator (ECV) 2000‒10000 EU/vial | 2mL × 10 vials/box |
BL5618B | Endotoxin Indicator (ECV) 2000‒10000 EU/vial | 10mL × 10 vials/box |
BL5619A | Endotoxin-Free Water | 8mL × 10 bottles/box |
BL5619B | Endotoxin-Free Water | 250mL |
BL5619C | Endotoxin-Free Water | 500mL |
The Reactive Oxygen Species (ROS) Assay Kit uses the fluorescent probe H2DCFDA to detect ROS levels. H2DCFDA is non-fluorescent and cell-permeable. Once inside cells, it is hydrolyzed by intracellular esterases to form DCFH, which is membrane-impermeable and retained within cells. Intracellular ROS oxidize non-fluorescent DCFH to produce fluorescent DCF. Fluorescence intensity correlates with ROS levels, allowing assessment of ROS content and changes in live cells. Detection can be performed by flow cytometry or fluorescence microscopy, making it a classic method for ROS detection.
Cat. No. | Description | Packaging |
BL714A | ROS Assay Kit | 1000T |
BL714B | ROS Assay Kit | 5000T |
Total SOD Activity Assay Kits are available in three formats: NBT, WST-1, and WST-8. NBT is the traditional method, while WST-1 and WST-8 are improved versions with higher sensitivity and stability. All three methods share a similar principle: superoxide anions generated by xanthine oxidase (XO) react with the reagents to form water-soluble formazan dye. SOD inhibits this reaction by catalyzing the dismutation of superoxide anions, so SOD activity is inversely correlated with formazan production. SOD activity can thus be calculated by colorimetric analysis. These kits incorporate catalase and other components to effectively eliminate interference from hydrogen peroxide in routine samples. For instance, the presence of up to 0.1 mM hydrogen peroxide in SOD standard samples does not significantly affect detection results. All three kits are suitable for measuring SOD activity in various biological samples, including cell or tissue homogenate supernatants, whole blood, erythrocyte extracts, and serum, offering broad applicability.
Cat. No. | Description | Packaging |
BL901A | Total SOD Activity Assay Kit (NBT Method) | 100T |
BL902A | Total SOD Activity Assay Kit (WST-1 Method) | 100T |
BL903A | Total SOD Activity Assay Kit (WST-8 Method) | 100T |
Lipid Peroxidation (MDA) Assay Kit is based on the colorimetric reaction between malondialdehyde (MDA) and thiobarbituric acid (TBA), which produces a red product. It is used for quantitative detection of MDA in plasma, serum, urine, animal/plant tissues, or cell lysates, making it widely used for assessing lipid peroxidation levels. This kit can detect MDA concentrations ranging from as low as 1 μM to as high as 200 μM. MDA levels in plasma and serum are typically around 2‒4 μM, while urine levels range from approximately 5‒30 μM, all within the detection range of this kit. Plasma, serum, and urine samples can be directly tested.
Cat. No. | Description | Packaging |
BL904A/B | Lipid Peroxidation (MDA) Assay Kit | 100T/500T |
The Senescence β-Galactosidase Staining Kit detects cellular senescence by measuring the activity of senescence-associated β-galactosidase (SA-β-Gal). In senescent cells, SA-β-Gal is active at pH 6.0 and catalyzes the substrate X-Gal to produce a blue product, resulting in blue deposits in the cytoplasm. Stained cells or tissues can be easily observed under light microscopy. This kit is suitable for senescence detection in cultured cells and tissue sections. It specifically stains senescent cells and does not stain presenescent cells, quiescent cells, immortal cells, or tumor cells.
Cat. No. | Description | Packaging |
BL133A | Senescence β-Galactosidase Staining Kit | 100T |
The Autophagy Staining Detection Kit (MDC Method) uses monodansylcadaverine (MDC) as a fluorescent probe for rapid and convenient detection of autophagy. Autophagy is a critical physiological process in eukaryotes for intracellular material turnover. It involves the formation of autophagosomes, which can be specifically labeled by MDC, an acidophilic fluorescent dye. This kit is suitable for fluorescence-based autophagy staining in cultured cells and can also be used for FACS (Fluorescence Activated Cell Sorting) analysis to quantify autophagy. Also known as MDC Staining Solution, it can be combined with EB for double staining.
Cat. No. | Description | Packaging |
BL1139A | Autophagy Staining Detection Kit (MDC Method) | 100T |
The Caspase-3 Activity Assay Kit uses spectrophotometry to measure caspase-3 enzyme activity in cell or tissue lysates or purified caspase-3 samples. Caspases are a family of proteases that play a key role in apoptosis. Caspase-3, also known as CPP32, Yama, or apopain, belongs to the CED-3 subfamily and is a crucial enzyme in apoptosis, being the most extensively studied caspase in mammalian cells. This kit is based on the cleavage of the substrate Ac-DEVD-pNA by caspase-3, releasing yellow pNA (p-nitroaniline). Caspase-3 activity is determined by measuring absorbance.
Cat. No. | Description | Packaging |
BL1212A/B | Caspase-3 Activity Assay Kit | 20T/100T |
LDH Cytotoxicity Assay Kit is based on a diaphorase-catalyzed INT colorimetric reaction, used to measure LDH activity released during cytotoxicity or in other samples. This kit is suitable for routine LDH activity detection and is commonly used for cytotoxicity assays based on LDH release. It can also be used for cell proliferation and cytotoxicity detection by measuring total cellular LDH activity.
Cat. No. | Description | Packaging |
BL1405A/B | LDH Cytotoxicity Assay Kit | 100T/500T |
Mycoplasma Stain Assay Kit is a classic DNA fluorescent staining method for in situ detection of mycoplasma or other prokaryotes in cultured cells, primarily used to identify mycoplasma contamination. The principle relies on the fluorescent dye bisbenzimide (Hoechst 33258), which binds to A-T rich regions of DNA. Since mycoplasma DNA has a high A-T content (55%‒80%), it can be specifically stained and detected. In contaminated cultures, numerous uniformly sized blue fluorescent dots are visible around the cells, indicating mycoplasma contamination.
Cat. No. | Description | Packaging |
BL1116A | Mycoplasma Stain Assay Kit | 100T |
Mycoplasma Detection Kit (PCR Method) uses PCR to specifically detect mycoplasma contamination in cultured cells and biological materials such as cell culture supernatants, experimental animal secretions, and animal serum. The kit employs mixed primers designed against the conserved region of the mycoplasma 16S rRNA sequence, allowing direct use of cell culture medium as the PCR template for specific amplification of mycoplasma DNA. Detection can be completed within one hour, with high sensitivity capable of detecting as few as 20 copies of mycoplasma DNA.
Cat. No. | Description | Packaging |
BL1469A | Mycoplasma Detection Kit (PCR Method) | 50T |
Luciferase Reporter Gene Assay Kit is based on firefly luciferase (~61 kDa), which catalyzes the oxidation of luciferin to oxyluciferin in the presence of ATP, Mg²⁺, and oxygen, producing bioluminescence at ~560 nm. Renilla luciferase (~36 kDa) catalyzes the oxidation of coelenterazine to coelenteramide in the presence of oxygen, producing bioluminescence at ~480 nm. Bioluminescence can be measured using a luminometer or liquid scintillation counter. This system provides a highly sensitive and efficient method for gene expression analysis. Typically, the transcriptional regulatory element or 5′ promoter region of a gene of interest is cloned upstream of the luciferase reporter gene, or the 3′-UTR is cloned downstream, to construct a reporter plasmid. After transfection and appropriate treatment, cells are lysed, and luciferase activity is measured to assess transcriptional regulation. Renilla luciferase is commonly used as an internal control to normalize for variations in transfection efficiency and cell number.
Cat. No. | Description | Packaging |
BL555A/B | Dual-Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1604A/B | Bright-Luc Firefly Luciferase Reporter Gene Assay Kit (Pre-Mixed) | 100T/1000T |
BL1605A/B | Bright-Luc Firefly Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1606A/B | One-Luc Firefly Luciferase Reporter Gene Assay Kit (Pre-Mixed) | 100T/1000T |
BL1607A/B | One-Luc Firefly Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1608A/B | Renilla-Luc Renilla Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1609A/B | Dual-Luc Dual-Luciferase Reporter Gene Assay Kit (Pre-Mixed) | 100T/1000T |
BL1610A/B | Dual-Luc Dual-Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1611A/B | Firefly Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1613A/B | Firefly Luciferase Reporter Gene Cell Lysis Buffer | 10mL/100mL |
BL1614A/B | Firefly Luciferase Reporter Gene Cell Lysis Buffer (Enhanced) | 10mL/100mL |
BL1612A/B | Renilla Luciferase Reporter Gene Assay Kit | 100T/1000T |
BL1615A/B | Renilla Luciferase Reporter Gene Cell Lysis Buffer | 10mL/100mL |
BL1616A/B | Dual-Luciferase Reporter Gene Cell Lysis Buffer | 10mL/100mL |
BL5561A | Firefly Luciferase Reporter Gene Assay Kit (Enhanced) | 100T |
BL5561B | Firefly Luciferase Reporter Gene Assay Kit (Enhanced) | 1000T |
BL5562A | Firefly Luciferase Reporter Gene Assay Kit (Stable) | 100T |
BL5562B | Firefly Luciferase Reporter Gene Assay Kit (Stable) | 1000T |
BL5563A | Renilla Luciferase Reporter Gene Assay Kit | 100T |
BL5563B | Renilla Luciferase Reporter Gene Assay Kit | 1000T |
BL5564A | Dual-Luciferase Reporter Gene Assay Kit | 100T |
BL5564B | Dual-Luciferase Reporter Gene Assay Kit | 1000T |
β-Galactosidase Reporter Gene Assay Kit is used for β-galactosidase reporter gene detection. β-Galactosidase is a commonly used reporter gene, often co-transfected with luciferase reporters as an internal control to normalize for variations in transfection efficiency. This kit uses colorless ONPG as a substrate, which is catalyzed by β-galactosidase to produce yellow o-nitrophenol. Absorbance is measured at 420 nm using a microplate reader or spectrophotometer to determine β-galactosidase activity.
Cat. No. | Description | Packaging |
BL1617A | β-Galactosidase Reporter Gene Assay Kit | 200T |
Mycoplasma Detection Kit (Chemiluminescent Method) is based on the activity of an enzyme unique to mycoplasma. This enzyme cleaves a specific substrate in the detection reagent while converting ADP to ATP. Luciferase then catalyzes luciferin oxidation in the presence of ATP, producing bioluminescence that can be measured by a luminometer to determine whether a sample is contaminated with mycoplasma. The assay requires only two steps and takes approximately 15 minutes. It offers high sensitivity by detecting metabolically active mycoplasma, making results more accurate than PCR-based methods.
Cat. No. | Description | Packaging |
BL1470A | Mycoplasma Detection Kit (Chemiluminescent Method) | 20T |
Mycoplasma Detection Kit (Isothermal Amplification) uses isothermal amplification with visual color change to rapidly detect 8 common mycoplasma species in cell culture media. No DNA extraction is required; simply add 1 μL of cell culture supernatant to the reaction mixture and incubate at 65°C for 30 min. Positive samples change from yellow to red. The process requires no PCR instrument or post-amplification electrophoresis, effectively preventing cross-contamination from aerosols. Compared to traditional PCR, this kit is simple to operate, highly sensitive, and tolerates various PCR inhibitors present in culture media, eliminating false negatives. Results show high consistency with qPCR.
Cat. No. | Description | Packaging |
BL1471A | One-Step Rapid Mycoplasma Detection Kit (Isothermal Amplification) | 50T |
NAD⁺/NADH Assay Kit uses WST-8 colorimetric reaction to measure NAD⁺ (oxidized nicotinamide adenine dinucleotide) and NADH (reduced nicotinamide adenine dinucleotide) levels, ratio, and total amount in cells, tissues, or other samples. The kit specifically detects NAD⁺ and NADH without interference from NADP⁺ and NADPH. During the reaction, NAD⁺ is reduced to NADH, which then reduces WST-8 to orange-yellow formazan with maximum absorbance at ~450 nm. The amount of formazan produced is proportional to the total amount of NAD⁺ or NADH in the sample.
Cat. No. | Description | Packaging |
BL1431A | NAD+/NADH Assay Kit | 100T |
NADP⁺/NADPH Assay Kit uses WST-8 colorimetric reaction to measure NADP⁺ (nicotinamide adenine dinucleotide phosphate, oxidized) and NADPH (reduced) levels, ratio, and total amount in cells, tissues, or other samples. The kit specifically detects NADP⁺ and NADPH without interference from NAD⁺ and NADH. During reaction, NADP⁺ is reduced to NADPH, which then reduces WST-8 to orange-yellow formazan with maximum absorbance at ~450 nm. Formazan production is proportional to total NADP⁺ or NADPH in sample.
Cat. No. | Description | Packaging |
BL1432A | NADP+/NADPH Assay Kit | 100T |
ATP Assay Kit is based on firefly luciferase catalyzing luciferin to produce fluorescence, which requires ATP for energy. When both firefly luciferase and luciferin are in excess, fluorescence production is proportional to ATP concentration within a certain range, enabling highly sensitive detection of ATP levels in solution.
Cat. No. | Description | Packaging |
BL1554A | ATP Assay Kit (1nM) | 200T |
BL1555A | Enhanced ATP Assay Kit (0.1nM) | 200T |
CellTiter-Flu Luminescent Cell Viability Assay Kit is based on luciferase catalyzing luciferin, ATP, and O₂ in the presence of Mg²⁺, converting chemical energy into light. In this reaction, ATP concentration is linearly correlated with luminescence intensity within a certain range. Luminescence signal is proportional to ATP amount, which is directly proportional to cell number in culture.
Cat. No. | Description | Packaging |
BL1618A | CellTiter-Flu Luminescent Cell Viability Assay Kit | 100T |
BL1618B | CellTiter-Flu Luminescent Cell Viability Assay Kit | 1000T |
BL1618C | CellTiter-Flu Luminescent Cell Viability Assay Kit | 5000T |
Hydrogen Peroxide Assay Kit is based on the oxidation of ferrous ions to ferric ions by hydrogen peroxide, which then form a purple complex with xylenol orange in a specific solution, enabling determination of hydrogen peroxide concentration. This improved formulation can detect as low as 1 μmol/L hydrogen peroxide.
Cat. No. | Description | Packaging |
BL1556A | Hydrogen Peroxide Assay Kit | 150T |
Glutathione Assay Kit uses glutathione reductase to reduce oxidized glutathione (GSSG) to reduced glutathione (GSH). GSH then reacts with the chromogenic substrate DTNB, producing yellow TNB and regenerating GSSG. Under appropriate reaction conditions, total glutathione (GSSG+GSH) becomes the rate-limiting factor for color development, with TNB formation proportional to total glutathione amount. Total glutathione is determined by measuring absorbance at 412 nm.To measure GSSG specifically, GSH is first removed from the sample using a selective reagent, then the same reaction is performed. GSH content is calculated by subtracting GSSG from total glutathione.
Cat. No. | Description | Packaging |
BL1557A | GSH and GSSG Assay Kit | 100T |
BL1558A | Total Glutathione Assay Kit | 100T |
Calcium Colorimetric Assay Kit is a colorimetric method for rapid quantitative detection of calcium ion levels in cells, tissues, serum, plasma, urine, culture media, and other samples. The principle is based on the reaction of calcium ions with o-cresolphthalein complexone under alkaline conditions, forming a purple complex. Calcium concentration is determined by measuring absorbance at 575 nm.
Cat. No. | Description | Packaging |
BL1561A | Calcium Colorimetric Assay Kit | 200T |
Alkaline Phosphatase Assay Kit provides a highly sensitive, simple, and direct method for detecting endogenous alkaline
phosphatase (ALP) activity in cell or tissue lysates, homogenates, plasma, serum, urine, and other samples. The principle is based on
p-nitrophenyl phosphate (pNPP) as a substrate. Under alkaline conditions, ALP cleaves pNPP to produce p-nitrophenol, which forms a
deep yellow quinoid structure with maximum absorbance at 405 nm. Higher color intensity indicates higher ALP activity.
Cat. No. | Description | Packaging |
BL1428A | Alkaline Phosphatase Assay Kit | 100T |
Total Antioxidant Capacity (T-AOC) Assay Kit (ABTS Method) uses ABTS as a chromogenic agent to measure total
antioxidant capacity in plasma, serum, saliva, urine, cell or tissue lysates, plant or herbal extracts, and various antioxidant solutions.
The principle is based on ABTS being oxidized to green ABTS⁺ by appropriate oxidants. Antioxidants inhibit ABTS⁺ formation, and total
antioxidant capacity is determined by measuring absorbance at 734 nm or 405 nm.
Cat. No. | Description | Packaging |
BL1429A | Total Antioxidant Capacity Assay Kit (ABTS Method) | 300T |
Acridine Orange (AO)/PI Dual Fluorescence Staining Kit uses AO/PI dual fluorescence counting to determine cell
concentration and viability. The staining solution contains acridine orange (green fluorescent dye) and propidium iodide (red
fluorescent dye). PI is membrane-impermeable and only enters cells with damaged membranes, while AO penetrates all cells. When
both dyes are present in the nucleus, PI causes reduction in AO fluorescence through fluorescence resonance energy transfer (FRET).
Therefore, intact nucleated cells stain green and are counted as viable, while damaged nucleated cells stain red and are counted as
non-viable.
Cat. No. | Description | Packaging |
BL5565A | Acridine Orange (AO)/PI Dual Fluorescence Staining Kit | 2×5mL |
BL5565B | Acridine Orange (AO)/PI Dual Fluorescence Staining Kit | 2×25mL |
Bacterial Endotoxin Detection Kit (Kinetic Chromogenic Method) is based on bacterial endotoxin specifically
activating factor C in the kit's main reagent. Activated factor C activates factor B, which then activates proclotting enzyme. The
activated clotting enzyme hydrolyzes a chromogenic substrate, releasing free pNA (p-nitroaniline) and causing changes in absorbance.
Endotoxin concentration is quantified by dynamically measuring the rate of absorbance change. It is used for quantitative determination
of bacterial endotoxin in human and animal injectable drugs, biologics, medical devices, and other samples.
Cat. No. | Description | Packaging |
BL5615A | Bacterial Endotoxin Detection Kit (Kinetic Chromogenic Method) 0.01‒10 EU/mL | 96T |
BL5616A | Bacterial Endotoxin Detection Kit (Kinetic Chromogenic Method) 0.005‒10 EU/mL | 96T |
Bacterial Endotoxin Detection Reagent (Gel Clot Method) is a freeze-dried product derived from the amebocyte
lysate of horseshoe crabs, containing proclotting enzyme and coagulogen that can be activated by trace amounts of bacterial
endotoxin. Under suitable conditions (temperature, pH, and absence of interfering substances), bacterial endotoxin activates the
proclotting enzyme in the reagent, causing a clotting reaction that forms a gel. The firmness of the gel determines the limit detection
of bacterial endotoxin. This product appears as a white or off-white lyophilized powder that is readily soluble in water. It is used for
limit testing of bacterial endotoxin in culture media, syringes, dialysis equipment, vaccines, antibiotics, protein drugs, and other
reagents, consumables, and pharmaceuticals.
Cat. No. | Description | Packaging |
BL5610A | Bacterial Endotoxin Detection Reagent (Gel Clot Method) 0.03 EU/mL | 10tests × 10vials/box |
BL5611A | Bacterial Endotoxin Detection Reagent (Gel Clot Method) 0.06 EU/mL | 10tests × 10vials/box |
BL5612A | Bacterial Endotoxin Detection Reagent (Gel Clot Method) 0.125 EU/mL | 10tests × 10vials/box |
BL5613A | Bacterial Endotoxin Detection Reagent (Gel Clot Method) 0.25 EU/mL | 10tests × 10vials/box |
BL5614A | Bacterial Endotoxin Detection Reagent (Gel Clot Method) 0.5 EU/mL | 10tests × 10vials/box |
BL5617A | Bacterial Endotoxin Working Standard (CSE) 10‒100 EU/vial | 10vials/box |
BL5618A | Endotoxin Indicator (ECV) 2000‒10000 EU/vial | 2mL × 10 vials/box |
BL5618B | Endotoxin Indicator (ECV) 2000‒10000 EU/vial | 10mL × 10 vials/box |
BL5619A | Endotoxin-Free Water | 8mL × 10 bottles/box |
BL5619B | Endotoxin-Free Water | 250mL |
BL5619C | Endotoxin-Free Water | 500mL |
