The Mitochondrial Membrane Potential Assay Kit (JC-1/JC-10) uses JC-1 or JC-10 as fluorescent probes to rapidly and sensitively detect changes in mitochondrial membrane potential (ΔΨm) in cells, tissues, or isolated mitochondria, making it suitable for early apoptosis detection. When ΔΨm is high, JC-1 or JC-10 aggregates in the mitochondrial matrix, forming red-fluorescent polymers. When ΔΨm is low, the probes remain as monomers that emit green fluorescence. This fluorescence shift provides a convenient way to monitor ΔΨm changes. The ratio of red to green fluorescence is commonly used to quantify mitochondrial depolarization. Compared to JC-1, JC-10 has better water solubility and may perform better in experiments requiring high dye concentrations.
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The figure shows JC-1 staining: normal cells on the left, treated cells on the right. | The figure shows JC-10 staining: normal cells on the left, treated cells on the right. | |||
Cat. No. | Description | Packaging |
BL711A | Mitochondrial Membrane Potential Assay Kit (JC-1) | 100T |
BL726A | Mitochondrial Membrane Potential Assay Kit (JC-10) | 100T |
BL1213A | Enhanced Mitochondrial Membrane Potential Assay Kit (JC-1) | 100T |
The Mitochondrial Membrane Potential Assay Kit (Rhodamine 123) uses Rhodamine 123 as a fluorescent probe to rapidly and sensitively detect changes in mitochondrial membrane potential (ΔΨm) in cells, tissues, or isolated mitochondria. In normal cells, Rhodamine 123 enters the mitochondrial matrix in a ΔΨm-dependent manner and emits bright yellow-green fluorescence. During apoptosis or necrosis, ΔΨm is lost and the mitochondrial permeability transition pore remains open, causing the collapse of ΔΨm. This leads to the release of Rhodamine 123 from mitochondria and a significant decrease in intracellular yellow-green fluorescence. After staining, cells can be analyzed by fluorescence microscopy, flow cytometry, or fluorescence microplate readers. Changes in fluorescence intensity reflect alterations in ΔΨm and indicate the occurrence of apoptosis or necrosis.
The figure shows Rhodamine 123 staining: normal cells on the left,
treated cells on the right.
Cat. No. | Description | Packaging |
BL778A | Mitochondrial Membrane Potential Assay Kit (Rhodamine 123) | 100-1000T |
BL931A | Rhodamine 123 | 5mg |
The Mitochondrial Permeability Transition Pore (MPTP) Detection Kit uses the membrane-permeable fluorescent probe Calcein AM to assess MPTP opening. Compared to methods based on mitochondrial membrane potential, this approach provides a more direct measurement of MPTP opening. Calcein AM exhibits low toxicity and does not inhibit cell proliferation, chemotaxis, or most other cellular functions, making it well-suited for live cell applications.
Cat. No. | Description | Packaging |
BL928A | Mitochondrial Permeability Transition Pore (MPTP) Detection Kit | 100-1000T |
The Mitochondrial Membrane Potential Assay Kit (JC-1/JC-10) uses JC-1 or JC-10 as fluorescent probes to rapidly and sensitively detect changes in mitochondrial membrane potential (ΔΨm) in cells, tissues, or isolated mitochondria, making it suitable for early apoptosis detection. When ΔΨm is high, JC-1 or JC-10 aggregates in the mitochondrial matrix, forming red-fluorescent polymers. When ΔΨm is low, the probes remain as monomers that emit green fluorescence. This fluorescence shift provides a convenient way to monitor ΔΨm changes. The ratio of red to green fluorescence is commonly used to quantify mitochondrial depolarization. Compared to JC-1, JC-10 has better water solubility and may perform better in experiments requiring high dye concentrations.
| ||||
The figure shows JC-1 staining: normal cells on the left, treated cells on the right. | The figure shows JC-10 staining: normal cells on the left, treated cells on the right. | |||
Cat. No. | Description | Packaging |
BL711A | Mitochondrial Membrane Potential Assay Kit (JC-1) | 100T |
BL726A | Mitochondrial Membrane Potential Assay Kit (JC-10) | 100T |
BL1213A | Enhanced Mitochondrial Membrane Potential Assay Kit (JC-1) | 100T |
The Mitochondrial Membrane Potential Assay Kit (Rhodamine 123) uses Rhodamine 123 as a fluorescent probe to rapidly and sensitively detect changes in mitochondrial membrane potential (ΔΨm) in cells, tissues, or isolated mitochondria. In normal cells, Rhodamine 123 enters the mitochondrial matrix in a ΔΨm-dependent manner and emits bright yellow-green fluorescence. During apoptosis or necrosis, ΔΨm is lost and the mitochondrial permeability transition pore remains open, causing the collapse of ΔΨm. This leads to the release of Rhodamine 123 from mitochondria and a significant decrease in intracellular yellow-green fluorescence. After staining, cells can be analyzed by fluorescence microscopy, flow cytometry, or fluorescence microplate readers. Changes in fluorescence intensity reflect alterations in ΔΨm and indicate the occurrence of apoptosis or necrosis.
The figure shows Rhodamine 123 staining: normal cells on the left,
treated cells on the right.
Cat. No. | Description | Packaging |
BL778A | Mitochondrial Membrane Potential Assay Kit (Rhodamine 123) | 100-1000T |
BL931A | Rhodamine 123 | 5mg |
The Mitochondrial Permeability Transition Pore (MPTP) Detection Kit uses the membrane-permeable fluorescent probe Calcein AM to assess MPTP opening. Compared to methods based on mitochondrial membrane potential, this approach provides a more direct measurement of MPTP opening. Calcein AM exhibits low toxicity and does not inhibit cell proliferation, chemotaxis, or most other cellular functions, making it well-suited for live cell applications.
Cat. No. | Description | Packaging |
BL928A | Mitochondrial Permeability Transition Pore (MPTP) Detection Kit | 100-1000T |
