During tissue cell isolation, culture, and passaging, cell dissociation reagents are typically used to disperse cells and prepare single-cell suspensions for subsequent subculturing, sampling, and analysis. Commonly used dissociation agents include trypsin, collagenase, and dispase. Trypsin is a serine protease that hydrolyzes intercellular proteins, thereby dissociating cells. Its activity is optimal at pH 8.0 and 37°C. Since different cell types respond differently to trypsin treatment, it is important to control the concentration, exposure time, and temperature to prevent over-digestion and cell damage.
Our trypsin cell digestion solutions are available in various concentrations. The commonly used working concentration is 0.25%. For semi-adherent cells or trypsin-sensitive cells, a concentration of 0.05% can be used directly, or other concentrations can be diluted with PBS to 0.05% to reduce cellular impact. The standard formulation includes EDTA to chelate Ca²⁺ and Mg²⁺, which could otherwise inhibit trypsin activity, ensuring effective cell dissociation. If EDTA may interfere with downstream experiments, an EDTA-free digestion solution is also available.
Cat. No. | Description | Packaging |
BL501A | Trypsin Cell Digestion Solution (0.25% Trypsin, with EDTA, without Phenol Red) | 100mL |
BL501B | Trypsin Cell Digestion Solution (0.25% Trypsin, with EDTA, without Phenol Red) | 500mL |
BL512A | Trypsin Cell Digestion Solution (0.25% Trypsin, with EDTA, with Phenol Red) | 100mL |
BL512B | Trypsin Cell Digestion Solution (0.25% Trypsin, with EDTA, with Phenol Red) | 500mL |
BL526A | Trypsin Cell Digestion Solution (0.25% Trypsin, without EDTA, without Phenol Red) | 100mL |
BL526B | Trypsin Cell Digestion Solution (0.25% Trypsin, without EDTA, without Phenol Red) | 500mL |
BL527A | Trypsin Cell Digestion Solution (0.25% Trypsin, without EDTA, with Phenol Red) | 100mL |
BL527B | Trypsin Cell Digestion Solution (0.25% Trypsin, without EDTA, with Phenol Red) | 500mL |
BL1093A | Trypsin Cell Digestion Solution (0.05% Trypsin, with EDTA, without Phenol Red) | 100mL |
BL1094A | Trypsin Cell Digestion Solution (0.05% Trypsin, with EDTA, with Phenol Red) | 100mL |
BL1095A | Trypsin Cell Digestion Solution (0.05% Trypsin, without EDTA, with Phenol Red) | 100mL |
BL1096A | Trypsin Cell Digestion Solution (2.5% Trypsin, without EDTA, without Phenol Red) | 100mL |
During tissue cell isolation, culture, and passaging, cell dissociation reagents are typically used to disperse cells and prepare single-cell suspensions for subsequent subculturing, sampling, and analysis. Commonly used dissociation agents include trypsin, collagenase, and dispase. Trypsin is a serine protease that hydrolyzes intercellular proteins, thereby dissociating cells. Its activity is optimal at pH 8.0 and 37°C. Since different cell types respond differently to trypsin treatment, it is important to control the concentration, exposure time, and temperature to prevent over-digestion and cell damage.
Our trypsin cell digestion solutions are available in various concentrations. The commonly used working concentration is 0.25%. For semi-adherent cells or trypsin-sensitive cells, a concentration of 0.05% can be used directly, or other concentrations can be diluted with PBS to 0.05% to reduce cellular impact. The standard formulation includes EDTA to chelate Ca²⁺ and Mg²⁺, which could otherwise inhibit trypsin activity, ensuring effective cell dissociation. If EDTA may interfere with downstream experiments, an EDTA-free digestion solution is also available.
Cat. No. | Description | Packaging |
BL501A | Trypsin Cell Digestion Solution (0.25% Trypsin, with EDTA, without Phenol Red) | 100mL |
BL501B | Trypsin Cell Digestion Solution (0.25% Trypsin, with EDTA, without Phenol Red) | 500mL |
BL512A | Trypsin Cell Digestion Solution (0.25% Trypsin, with EDTA, with Phenol Red) | 100mL |
BL512B | Trypsin Cell Digestion Solution (0.25% Trypsin, with EDTA, with Phenol Red) | 500mL |
BL526A | Trypsin Cell Digestion Solution (0.25% Trypsin, without EDTA, without Phenol Red) | 100mL |
BL526B | Trypsin Cell Digestion Solution (0.25% Trypsin, without EDTA, without Phenol Red) | 500mL |
BL527A | Trypsin Cell Digestion Solution (0.25% Trypsin, without EDTA, with Phenol Red) | 100mL |
BL527B | Trypsin Cell Digestion Solution (0.25% Trypsin, without EDTA, with Phenol Red) | 500mL |
BL1093A | Trypsin Cell Digestion Solution (0.05% Trypsin, with EDTA, without Phenol Red) | 100mL |
BL1094A | Trypsin Cell Digestion Solution (0.05% Trypsin, with EDTA, with Phenol Red) | 100mL |
BL1095A | Trypsin Cell Digestion Solution (0.05% Trypsin, without EDTA, with Phenol Red) | 100mL |
BL1096A | Trypsin Cell Digestion Solution (2.5% Trypsin, without EDTA, without Phenol Red) | 100mL |
