In different cell culture experiments, specific nutritional supplements are sometimes added to achieve optimal growth conditions for the cultured cells, or certain components are introduced to influence cells and produce specific effects.
HAT and HT medium supplements mainly consist of hypoxanthine, aminopterin, and thymidine. Under normal conditions, cells have two pathways for purine and pyrimidine synthesis: the de novo synthesis pathway and the salvage pathway. Aminopterin acts as a dihydrofolate analog that blocks the de novo biosynthesis of purines and pyrimidines. Only cells capable of utilizing the salvage pathway can survive. Therefore, HAT/HT medium supplements can be used for the selection of specific cells, such as the selection and validation of hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient clones, the production of monoclonal antibodies (mAbs), and luciferase-based bioassays (LUC).
Cat. No. | Description | Packaging |
BL317A | HT Medium Supplement (50×) | 10mL |
BL318A | HAT Medium Supplement (50×) | 10mL |
BL1313A | HT Medium Supplement Solution (50×) | 10mL |
BL1313B | HT Medium Supplement Solution (50×) | 10×10mL |
BL1314A | HAT Medium Supplement Solution (50×) | 10mL |
BL1314B | HAT Medium Supplement Solution (50×) | 10×10mL |
B-27 and N-2 supplements are commonly used to promote the survival and maturation of neurons. During neuronal cell culture, because neurons are highly sensitive and fragile, cell death can easily occur. Extensive research has shown that N-2 supplement supports the growth, differentiation, survival, and expression of post-mitotic neurons in culture. It promotes the expression of neuron-specific genes while inhibiting the growth of non-neuronal cells. B-27 supplement is particularly effective in supporting the long-term maintenance of neurons. Both supplements play essential and indispensable roles in neuronal cell culture.
Cat. No. | Description | Packaging |
BL319A | B-27 Serum-Free Supplement (50×) | 10mL |
BL320A | N-2 Serum-Free Supplement (100×) | 5mL |
BL321A | B-27 Serum-Free Supplement without Vitamin A (50×) | 10mL |
L-Glutamine (L-Gln), also known as 2-amino-4-carbamoylbutanoic acid, is the amide of glutamic acid. L-Glutamine is a coding amino acid in protein synthesis and is classified as a non-essential amino acid in mammals, as it can be synthesized from glucose in the body. With a molecular weight of 161.15, it is widely used as a supplement in mammalian cell culture media. L-Glutamine in powder form or frozen solutions is very stable, but it degrades relatively quickly in liquid media or stock solutions. Therefore, fresh L-glutamine supplementation is often required before use to achieve optimal cell performance. This product has a concentration of 200 mM. The solution has been filter-sterilized through a 0.22 μm membrane and is ready for direct use in cell culture and other applications; no additional filtration or sterilization is required before use.
Cat. No. | Description | Packaging |
BL549A | L-Glutamine Solution 100× | 100mL |
Sodium Pyruvate is a commonly used supplement in cell culture. In addition to glucose, sodium pyruvate can serve as an energy source and is often added to cell culture media as an alternative carbon source. It also helps prevent the accumulation of lactate, a metabolic byproduct that occurs when glucose is the sole carbon source. Sodium pyruvate can be added during clonal culture or when reducing serum concentration. Additionally, it helps mitigate phototoxicity induced by fluorescent substances.
Cat. No. | Description | Packaging |
BL1277A | Sodium Pyruvate Solution (100 mM) | 100mL |
Non-Essential Amino Acids (NEAA) are a mixed solution of seven non-essential amino acids (glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline, and L-serine). They are a common supplement in cell culture, primarily reducing the metabolic burden on cells to produce these amino acids themselves. NEAA promote cell proliferation and metabolism, thereby enhancing cell viability. They are typically used in combination with MEM basal medium but can also be added to other culture media at an appropriate ratio based on specific experimental needs. This product is a 100× concentrated solution. It should be added to the culture medium according to the required volume.
Cat. No. | Description | Packaging |
BL1115A | Non-Essential Amino Acids Solution (NEAA, 100×) | 100mL |
ITS Medium Supplement (Insulin-Transferrin-Selenium Medium Supplement) is a supplement for basal cell culture media. This product can significantly reduce the amount of fetal bovine serum required in various cell culture processes and is commonly used in serum-free or low-serum culture systems. Typically, it can reduce fetal bovine serum usage from 10% to 2‒4%. ITS-A Medium Supplement and ITS-X Medium Supplement are based on ITS Medium Supplement, with the addition of sodium pyruvate and ethanolamine, respectively.
Cat. No. | Description | Packaging |
BL1886A | ITS Medium Supplement (100×) | 10mL |
BL1887A | ITS-A Medium Supplement (100×) | 10mL |
BL1888A | ITS-X Medium Supplement (100×) | 10mL |
Dexamethasone is a synthetic glucocorticoid, has a molecular weight of 392.5 and CAS number 50-02-2. It is a corticosteroid and an anti-inflammatory glucocorticoid. Dexamethasone has a range of effects on cell survival, cell signal transduction, and gene expression. For example, it inhibits the induction of nitric oxide synthase (IC50 = 5 nM); enhances cation transport in aortic smooth muscle cells by stimulating the Na⁺-K⁺ pump; reduces the activity of cyclin A and Cdk2; inhibits G1/S transition in osteoblasts; suppresses Rb protein phosphorylation; and induces apoptosis in human thymocytes. This product is an alcohol-soluble solution at a concentration of 1 mg/mL, filter-sterilized, and is typically used after 100-fold dilution.
Cat. No. | Description | Packaging |
BL171A | Dexamethasone Solution (100×, 1 mg/mL) | 10mL |
BL171B | Dexamethasone Solution (100×, 1 mg/mL) | 50mL |
Lipopolysaccharide (LPS) Solution is a ready-to-use aqueous solution of LPS derived from *Escherichia coli* 055:B5. Lipopolysaccharide (LPS) is a characteristic component of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system via Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family that recognizes common pathogen- associated molecular patterns (PAMPs). This product is used for in vitro culture of B cells, monocytes, macrophages, and other cells that respond to LPS.
Cat. No. | Description | Packaging |
BL1386A | Lipopolysaccharide (LPS) Solution O55:B5，1mg/mL | 0.5mL |
Basement Membrane Matrix is a basement membrane matrix extracted from Engelbreth-Holm-Swarm (EHS) mouse tumors, which are rich in extracellular matrix proteins. Its main component is laminin, along with type IV collagen, heparan sulfate proteoglycans, entactin, various growth factors, tissue plasminogen activator, and trace amounts of matrix metalloproteinases. It is widely used in research areas such as angiogenesis, tumor cell migration, in vivo tumor model establishment, 3D tumor cell models, and organoid culture.
Our Basement Membrane Matrix product line includes standard formulations with or without phenol red, low growth factor formulations, stem cell-specific formulations, and organoid-specific formulations. Phenol red-free Basement Membrane Matrix is suitable for applications such as fluorescence detection or colorimetric assays. All products are free of lactate dehydrogenase elevating virus (LDEV) and other mouseor tumor-derived viruses. The appropriate Basement Membrane Matrix formulation can be selected based on experimental needs.
Type | Description | Application Scenarios | Cat. No. | Packaging |
Standard Type | LDEV-free, with phenol red | Used for routine cell experiments such as 2D and 3D cell culture, cell growth, differentiation, morphology studies, cell function, angiogenesis, cell migration/invasion, gene/protein expression, with phenol red as a pH indicator. | BL1833A | 1mL |
BL1833B | 5mL | |||
BL1833C | 10mL | |||
LDEV-free, without phenol red | Used for routine cell experiments such as 2D and 3D cell culture, cell growth, differentiation, morphology studies, cell function, angiogenesis, cell migration/invasion, gene/protein expression; without phenol red to prevent interference. | BL1834A | 1mL | |
BL1834B | 5mL | |||
BL1834C | 10mL | |||
Low Growth Factor | LDEV-free, with phenol red | Reduces background interference from growth factors, suitable for studies on cell signaling pathways and cytokines, with phenol red as a pH indicator. | BL1835A | 1mL |
BL1835B | 5mL | |||
BL1835C | 10mL | |||
LDEV-free, without phenol red | Reduces background interference from growth factors, suitable for studies on cell signaling pathways and cytokines; without phenol red to prevent interference. | BL1836A | 1mL | |
BL1836B | 5mL | |||
BL1836C | 10mL | |||
Stem Cell-Specific | LDEV-free, without phenol red | Stem cell culture, such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC), providing the reproducibility and consistency required for feeder-free culture of hESC and iPSC. | BL1837A | 1mL |
BL1837B | 5mL | |||
BL1837C | 10mL | |||
Organoid Specific | LDEV-free, without phenol red | Suitable for organoid research, providing necessary growth factors, proteins, and matrix structure for organoid growth and differentiation studies. | BL1838A | 1mL |
BL1838B | 5mL | |||
BL1838C | 10mL | |||
High Concentration | LDEV-free, with phenol red | The high protein concentration provides increased matrix stiffness and scaffold integrity. Suitable for in vivo tumor formation and in vivo angiogenesis assays, with phenol red as a pH indicator. | BL5597A | 1mL |
BL5597B | 5mL | |||
BL5597C | 10mL | |||
LDEV-free, without phenol red | The high protein concentration provides increased matrix stiffness and scaffold integrity. Suitable for in vivo tumor formation and in vivo angiogenesis assays; without phenol red to prevent interference. | BL5598A | 1mL | |
BL5598B | 5mL | |||
BL5598C | 10mL | |||
High Concentration Low Growth Factor | LDEV-free, with phenol red | Designed for in vivo applications requiring both high protein concentration and reduced growth factor influence, with phenol red as a pH indicator. | BL5599A | 1mL |
BL5599B | 5mL | |||
BL5599C | 10mL | |||
LDEV-free, without phenol red | Designed for in vivo applications requiring both high protein concentration and reduced growth factor influence; without phenol red to prevent interference. | BL5600A | 1mL | |
BL5600B | 5mL | |||
BL5600C | 10mL |
3D Cell Culture Coating Solution is a convenient and efficient solution for preparing 96-well U-bottom plates for 3D cell culture, supporting sustained spheroid formation and culture. This product is a special high-molecular polymer that rapidly forms a hydrophilic coating on the container surface to inhibit cell adhesion and growth. It provides a 3D growth microenvironment, allowing single-cell suspensions to continuously form spheroids, including tumor spheroids, tumor organoids, neurospheres, and leukocyte spheroids. Cell culture plates treated with this 3D coating solution generate relatively uniform spheroids and are widely used in tumor research, drug screening, organoid construction, tissue engineering, and related fields.
Cat. No. | Description | Packaging |
BL5139A | 3D Cell Culture Coating Solution | 20mL |
BL5139B | 3D Cell Culture Coating Solution | 100mL |
BL5140A | 3D Cell Culture Coating Kit | Kit |
In different cell culture experiments, specific nutritional supplements are sometimes added to achieve optimal growth conditions for the cultured cells, or certain components are introduced to influence cells and produce specific effects.
HAT and HT medium supplements mainly consist of hypoxanthine, aminopterin, and thymidine. Under normal conditions, cells have two pathways for purine and pyrimidine synthesis: the de novo synthesis pathway and the salvage pathway. Aminopterin acts as a dihydrofolate analog that blocks the de novo biosynthesis of purines and pyrimidines. Only cells capable of utilizing the salvage pathway can survive. Therefore, HAT/HT medium supplements can be used for the selection of specific cells, such as the selection and validation of hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient clones, the production of monoclonal antibodies (mAbs), and luciferase-based bioassays (LUC).
Cat. No. | Description | Packaging |
BL317A | HT Medium Supplement (50×) | 10mL |
BL318A | HAT Medium Supplement (50×) | 10mL |
BL1313A | HT Medium Supplement Solution (50×) | 10mL |
BL1313B | HT Medium Supplement Solution (50×) | 10×10mL |
BL1314A | HAT Medium Supplement Solution (50×) | 10mL |
BL1314B | HAT Medium Supplement Solution (50×) | 10×10mL |
B-27 and N-2 supplements are commonly used to promote the survival and maturation of neurons. During neuronal cell culture, because neurons are highly sensitive and fragile, cell death can easily occur. Extensive research has shown that N-2 supplement supports the growth, differentiation, survival, and expression of post-mitotic neurons in culture. It promotes the expression of neuron-specific genes while inhibiting the growth of non-neuronal cells. B-27 supplement is particularly effective in supporting the long-term maintenance of neurons. Both supplements play essential and indispensable roles in neuronal cell culture.
Cat. No. | Description | Packaging |
BL319A | B-27 Serum-Free Supplement (50×) | 10mL |
BL320A | N-2 Serum-Free Supplement (100×) | 5mL |
BL321A | B-27 Serum-Free Supplement without Vitamin A (50×) | 10mL |
L-Glutamine (L-Gln), also known as 2-amino-4-carbamoylbutanoic acid, is the amide of glutamic acid. L-Glutamine is a coding amino acid in protein synthesis and is classified as a non-essential amino acid in mammals, as it can be synthesized from glucose in the body. With a molecular weight of 161.15, it is widely used as a supplement in mammalian cell culture media. L-Glutamine in powder form or frozen solutions is very stable, but it degrades relatively quickly in liquid media or stock solutions. Therefore, fresh L-glutamine supplementation is often required before use to achieve optimal cell performance. This product has a concentration of 200 mM. The solution has been filter-sterilized through a 0.22 μm membrane and is ready for direct use in cell culture and other applications; no additional filtration or sterilization is required before use.
Cat. No. | Description | Packaging |
BL549A | L-Glutamine Solution 100× | 100mL |
Sodium Pyruvate is a commonly used supplement in cell culture. In addition to glucose, sodium pyruvate can serve as an energy source and is often added to cell culture media as an alternative carbon source. It also helps prevent the accumulation of lactate, a metabolic byproduct that occurs when glucose is the sole carbon source. Sodium pyruvate can be added during clonal culture or when reducing serum concentration. Additionally, it helps mitigate phototoxicity induced by fluorescent substances.
Cat. No. | Description | Packaging |
BL1277A | Sodium Pyruvate Solution (100 mM) | 100mL |
Non-Essential Amino Acids (NEAA) are a mixed solution of seven non-essential amino acids (glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline, and L-serine). They are a common supplement in cell culture, primarily reducing the metabolic burden on cells to produce these amino acids themselves. NEAA promote cell proliferation and metabolism, thereby enhancing cell viability. They are typically used in combination with MEM basal medium but can also be added to other culture media at an appropriate ratio based on specific experimental needs. This product is a 100× concentrated solution. It should be added to the culture medium according to the required volume.
Cat. No. | Description | Packaging |
BL1115A | Non-Essential Amino Acids Solution (NEAA, 100×) | 100mL |
ITS Medium Supplement (Insulin-Transferrin-Selenium Medium Supplement) is a supplement for basal cell culture media. This product can significantly reduce the amount of fetal bovine serum required in various cell culture processes and is commonly used in serum-free or low-serum culture systems. Typically, it can reduce fetal bovine serum usage from 10% to 2‒4%. ITS-A Medium Supplement and ITS-X Medium Supplement are based on ITS Medium Supplement, with the addition of sodium pyruvate and ethanolamine, respectively.
Cat. No. | Description | Packaging |
BL1886A | ITS Medium Supplement (100×) | 10mL |
BL1887A | ITS-A Medium Supplement (100×) | 10mL |
BL1888A | ITS-X Medium Supplement (100×) | 10mL |
Dexamethasone is a synthetic glucocorticoid, has a molecular weight of 392.5 and CAS number 50-02-2. It is a corticosteroid and an anti-inflammatory glucocorticoid. Dexamethasone has a range of effects on cell survival, cell signal transduction, and gene expression. For example, it inhibits the induction of nitric oxide synthase (IC50 = 5 nM); enhances cation transport in aortic smooth muscle cells by stimulating the Na⁺-K⁺ pump; reduces the activity of cyclin A and Cdk2; inhibits G1/S transition in osteoblasts; suppresses Rb protein phosphorylation; and induces apoptosis in human thymocytes. This product is an alcohol-soluble solution at a concentration of 1 mg/mL, filter-sterilized, and is typically used after 100-fold dilution.
Cat. No. | Description | Packaging |
BL171A | Dexamethasone Solution (100×, 1 mg/mL) | 10mL |
BL171B | Dexamethasone Solution (100×, 1 mg/mL) | 50mL |
Lipopolysaccharide (LPS) Solution is a ready-to-use aqueous solution of LPS derived from *Escherichia coli* 055:B5. Lipopolysaccharide (LPS) is a characteristic component of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system via Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family that recognizes common pathogen- associated molecular patterns (PAMPs). This product is used for in vitro culture of B cells, monocytes, macrophages, and other cells that respond to LPS.
Cat. No. | Description | Packaging |
BL1386A | Lipopolysaccharide (LPS) Solution O55:B5，1mg/mL | 0.5mL |
Basement Membrane Matrix is a basement membrane matrix extracted from Engelbreth-Holm-Swarm (EHS) mouse tumors, which are rich in extracellular matrix proteins. Its main component is laminin, along with type IV collagen, heparan sulfate proteoglycans, entactin, various growth factors, tissue plasminogen activator, and trace amounts of matrix metalloproteinases. It is widely used in research areas such as angiogenesis, tumor cell migration, in vivo tumor model establishment, 3D tumor cell models, and organoid culture.
Our Basement Membrane Matrix product line includes standard formulations with or without phenol red, low growth factor formulations, stem cell-specific formulations, and organoid-specific formulations. Phenol red-free Basement Membrane Matrix is suitable for applications such as fluorescence detection or colorimetric assays. All products are free of lactate dehydrogenase elevating virus (LDEV) and other mouseor tumor-derived viruses. The appropriate Basement Membrane Matrix formulation can be selected based on experimental needs.
Type | Description | Application Scenarios | Cat. No. | Packaging |
Standard Type | LDEV-free, with phenol red | Used for routine cell experiments such as 2D and 3D cell culture, cell growth, differentiation, morphology studies, cell function, angiogenesis, cell migration/invasion, gene/protein expression, with phenol red as a pH indicator. | BL1833A | 1mL |
BL1833B | 5mL | |||
BL1833C | 10mL | |||
LDEV-free, without phenol red | Used for routine cell experiments such as 2D and 3D cell culture, cell growth, differentiation, morphology studies, cell function, angiogenesis, cell migration/invasion, gene/protein expression; without phenol red to prevent interference. | BL1834A | 1mL | |
BL1834B | 5mL | |||
BL1834C | 10mL | |||
Low Growth Factor | LDEV-free, with phenol red | Reduces background interference from growth factors, suitable for studies on cell signaling pathways and cytokines, with phenol red as a pH indicator. | BL1835A | 1mL |
BL1835B | 5mL | |||
BL1835C | 10mL | |||
LDEV-free, without phenol red | Reduces background interference from growth factors, suitable for studies on cell signaling pathways and cytokines; without phenol red to prevent interference. | BL1836A | 1mL | |
BL1836B | 5mL | |||
BL1836C | 10mL | |||
Stem Cell-Specific | LDEV-free, without phenol red | Stem cell culture, such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC), providing the reproducibility and consistency required for feeder-free culture of hESC and iPSC. | BL1837A | 1mL |
BL1837B | 5mL | |||
BL1837C | 10mL | |||
Organoid Specific | LDEV-free, without phenol red | Suitable for organoid research, providing necessary growth factors, proteins, and matrix structure for organoid growth and differentiation studies. | BL1838A | 1mL |
BL1838B | 5mL | |||
BL1838C | 10mL | |||
High Concentration | LDEV-free, with phenol red | The high protein concentration provides increased matrix stiffness and scaffold integrity. Suitable for in vivo tumor formation and in vivo angiogenesis assays, with phenol red as a pH indicator. | BL5597A | 1mL |
BL5597B | 5mL | |||
BL5597C | 10mL | |||
LDEV-free, without phenol red | The high protein concentration provides increased matrix stiffness and scaffold integrity. Suitable for in vivo tumor formation and in vivo angiogenesis assays; without phenol red to prevent interference. | BL5598A | 1mL | |
BL5598B | 5mL | |||
BL5598C | 10mL | |||
High Concentration Low Growth Factor | LDEV-free, with phenol red | Designed for in vivo applications requiring both high protein concentration and reduced growth factor influence, with phenol red as a pH indicator. | BL5599A | 1mL |
BL5599B | 5mL | |||
BL5599C | 10mL | |||
LDEV-free, without phenol red | Designed for in vivo applications requiring both high protein concentration and reduced growth factor influence; without phenol red to prevent interference. | BL5600A | 1mL | |
BL5600B | 5mL | |||
BL5600C | 10mL |
3D Cell Culture Coating Solution is a convenient and efficient solution for preparing 96-well U-bottom plates for 3D cell culture, supporting sustained spheroid formation and culture. This product is a special high-molecular polymer that rapidly forms a hydrophilic coating on the container surface to inhibit cell adhesion and growth. It provides a 3D growth microenvironment, allowing single-cell suspensions to continuously form spheroids, including tumor spheroids, tumor organoids, neurospheres, and leukocyte spheroids. Cell culture plates treated with this 3D coating solution generate relatively uniform spheroids and are widely used in tumor research, drug screening, organoid construction, tissue engineering, and related fields.
Cat. No. | Description | Packaging |
BL5139A | 3D Cell Culture Coating Solution | 20mL |
BL5139B | 3D Cell Culture Coating Solution | 100mL |
BL5140A | 3D Cell Culture Coating Kit | Kit |
